PXD060539

PXD060539 is an original dataset announced via ProteomeXchange.

Title	Identification of crucial genes sustaining the curled leaf phenotype in perennial transgenic poplar via advanced proteomic and phosphoproteomic analyses
Description	The perennial genetically modified Populus 741, exhibiting sustained overexpression of PtoCYCD3;3, consistently shows adaxial curvature and pronounced surface wrinkling. To investigate these morphological changes, TMT quantitative proteomics and phosphoproteomics were performed on leaves of transgenic and wild-type plants. Quantitative proteomics identified significant changes in protein abundance associated with photosynthesis, phytohormones, and cell proliferation. Notably, histone deacetylase 6 (HDA6), ANGUSTIFOLIA (AN), and cellulose synthase-like (CSL) proteins associated with leaf curling were significantly upregulated in transgenic poplar. Phosphoproteomics revealed enrichment of proteins such as HERK1, DGK, OST1, and BIG, which are involved in brassinosteroid (BR), abscisic acid (ABA), and other phytohormone signaling pathways. These analyses demonstrated the impact of exogenous gene PtoCYCD3;3 integration on photosynthetic pathways, hormone signaling, and cell proliferation, highlighting its role in modulating leaf morphogenesis in perennial Populus 741.
HostingRepository	iProX
AnnounceDate	2025-02-06
AnnouncementXML	Submission_2025-02-05_21:55:36.140.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhaoqun Wu
SpeciesList	scientific name: Populus tomentosa; NCBI TaxID: 118781;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-05 21:55:14	ID requested
⏵ 1	2025-02-05 21:55:36	announced

Dataset with its publication pending

submitter keyword: PtoCYCD3, Populus 741, leaves curled,Proteomics, Phosphoproteomics

Hailing Yang
contact affiliation	Beijing Forestry University
contact email	yhailing77@163.com
lab head
Zhaoqun Wu
contact affiliation	Beijing Forestry University
contact email	qunzhaowu@bjfu.edu.cn
dataset submitter

iProX dataset URI