PXD060532 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TAZ (WWTR1) Interactome Analysis using a GBP nanotrap based affinity purification approach |
Description | The transcriptional co-activator TAZ (WWTR1) plays a crucial role in regulating gene expression related to cell proliferation, differentiation, and tissue homeostasis, particularly within the Hippo signaling pathway. Since TAZ does not directly bind to DNA, it largely relies on its protein-protein interaction network to perform its functions, necessitating a detailed characterization of the TAZ interactome. To achieve this, a GFP-Nanotrap-based affinity purification approach combined with LC-MS/MS protein identification was employed to document a comprehensive list of both known and novel components of the TAZ interactome in myogenic cells. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-11 |
AnnouncementXML | Submission_2025-02-11_09:14:37.071.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | John McDermott |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-05 15:45:04 | ID requested | |
⏵ 1 | 2025-02-11 09:14:38 | announced | |
Publication List
Kelebeev J, MacKeracher A, Miyake T, McDermott JC, TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry. J Cell Sci, 138(4):(2025) [pubmed] |
10.1242/jcs.263527; |
Keyword List
submitter keyword: mass spectrometry, affinity-purification, skeletal muscle,TAZ, nanobody |
Contact List
John Mcdermott |
contact affiliation | York University |
contact email | jmcderm@yorku.ca |
lab head | |
John McDermott |
contact affiliation | York University |
contact email | jmcderm@yorku.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060532 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060532
- Label: PRIDE project
- Name: TAZ (WWTR1) Interactome Analysis using a GBP nanotrap based affinity purification approach