PXD060511
PXD060511 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Novel antimicrobial peptides and peptide-microbiome crosstalk in Appalachian salamander skin |
| Description | Host antimicrobial peptides (AMPs) are an ancient defense system found in all multicellular organisms that interact with host microbiomes. We used multi-omics and mathematical tools to discover new AMPs and examine AMP-microbial interactions in three Appalachian salamander species (Plethodon cinereus, Eurycea bislineata and Notophthalmus viridescens). We conducted skin transcriptomics (n = 13), proteomics (n = 91) and AMP database querying to identify candidate AMPs. With candidate AMPs, we identified correlations with the skin microbiome (16S rRNA amplicon) and synthesized 20 peptides to use in challenge assays with pathogens of amphibians (Batrachochytrium dendrobatidis: Bd) and humans (ESKAPEE pathogen panel). Using transcriptomics, candidate AMP genes (30-67 genes) were detected in all individuals with Cathelidicin-like peptides being most common. Using proteomics, detectable AMPs were only found in a subset of salamanders (31/91) - predominately E. bislineata - with Kinin-like peptides being most common. Candidate AMP composition generally predicted skin bacterial composition. Crude and synthesized peptides showed limited activity against Bd. Two synthesized Cathelicidin-like peptides (from P. cinereus) showed moderate to strong killing activity against human pathogens, Acinetobacter baumannii and Escherichia coli. We show that mining under-studied taxa and using multi-omics fuels AMPs discovery, reveals dynamic AMPs-microbial relationships, and informs the therapeutic potential of AMPs usage in conservation and translational applications. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-14 |
| AnnouncementXML | Submission_2025-10-14_04:51:34.091.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Timothy Cleland |
| SpeciesList | scientific name: Plethodon cinereus; common name: red-backed salamander; NCBI TaxID: 141976; scientific name: Eurycea bislineata; common name: two-lined salamander; NCBI TaxID: 134758; scientific name: Notophthalmus viridescens; common name: eastern newt; NCBI TaxID: 8316; |
| ModificationList | Oxidation |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-02-05 04:51:26 | ID requested | |
| ⏵ 1 | 2025-10-14 04:51:34 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Salamander, Antimicrobial peptides, DatasetType:Proteomics |
Contact List
| Timothy Cleland | |
|---|---|
| contact affiliation | Smithsonian Institution |
| contact email | clelandtp@si.edu |
| lab head | |
| Carly Muletz-Wolz | |
| contact affiliation | Smithsonian Institution |
| contact email | MuletzC@si.edu |
| lab head | |
| Timothy Cleland | |
| contact affiliation | Smithsonian Institution |
| contact email | clelandtp@si.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097047/ |
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