PXD060506

PXD060506 is an original dataset announced via ProteomeXchange.

Title	Εthylene response factor ERF1A positively regulates the UV-C delayed ripening in peach fruit
Description	The ripening of climacteric fruits, such as peach, is a complex process regulated by internal signals and external stimuli. Ultraviolet-C (UV-C) irradiation has been shown to delay fruit ripening, yet the mechanisms underlying this phenomenon remain poorly understood. RNA interference (RNAi)-mediated silencing of ERF1A supported its crucial role in UV-C-mediated ripening delay, as evidenced by changes in ethylene production, ripening-associated metabolites, fruit softening and the fluorescent immunolocalization of cell wall matrix components, particularly arabinogalactan, pectin and xyloglucan epitopes. Levels of auxin and salicylic acid were increased while abscisic acid was reduced in ERF1A silencing peels. Suppression of ERF1A altered the biosynthesis of peel-derived volatile compounds. Proteomic analysis identified several ERF1A-target genes and highlighted that the UV-C delayed ripening could be reversed by ERF1A silencing. Evidence is also presented supporting that PpCXE11, PpCXE13 and PpSABP2 are target of ERF1A transcription. These findings provide insights into the mechanisms driving UV-C-induced ripening delay in peach and underscore the central role of ERF1A in modulating this process.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_16:40:32.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Prunus persica; NCBI TaxID: NEWT:3760;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-04 23:54:28	ID requested
⏵ 1	2025-10-12 16:40:33	announced

10.1093/plphys/kiaf409;

Nasiopoulou E, Michailidis M, Skodra C, Adamakis IS, Samiotaki M, Tanou G, Bazakos C, Dalakouras A, Molassiotis A, The ethylene response factor ERF1A regulates UV-C-induced delayed ripening in peach fruit. Plant Physiol, 199(2):(2025) [pubmed]

submitter keyword: ripening delay, RNA interference,Ethylene response factor, peel tissue, UV-C irradiation

Athanassios Molassiotis
contact affiliation	Laboratory of Pomology, Department of Horticulture, Aristotle University of Thessaloniki, 57001 Thessaloniki-Thermi, Greece
contact email	amolasio@agro.auth.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD060506

PRIDE project URI

• PRIDE
  1. PXD060506
     1. Label: PRIDE project
     2. Name: Εthylene response factor ERF1A positively regulates the UV-C delayed ripening in peach fruit