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PXD060500

PXD060500 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA rapid, robust, and refined solution Enhance Accuracy and Throughput in Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies
DescriptionElucidating antibody sequences is crucial for understanding antigen binding and advancing therapeutic and research applications. However, current de novo sequencing technology is hindered by high costs, low throughput, missing ion information, and assembly limitations in accuracy and robustness. Here, we developed a refined sequencing solution by integrating Single-Pot Multi-Enzymatic Gradient Digestion (SP-MEGD) with a beam search-based assembler (Fusion) to decipher complete sequences. This solution achieved high accuracy (over 99%) in antibody reconstruction. Biological validation of multiple commercial antibodies with unavailable sequences further supports its effectiveness. Additionally, our solution can discriminate and achieve over 99% accurate sequence coverage of mixtures containing two or three COVID-19 neutralizing antibodies. To our knowledge, this is the first study to enable simultaneous sequencing of multiple antibodies using only bottom-up mass spectrometry, improving throughput without increasing duration or expense. In summary, our method advances antibody research by enhancing the accuracy and efficiency of complex antibody sequencing.
HostingRepositoryiProX
AnnounceDate2025-02-05
AnnouncementXMLSubmission_2025-02-04_22:44:06.848.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYueting Xiong
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListdeaminated residue; oxidation; deamidated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-04 22:43:43ID requested
12025-02-04 22:44:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mass spectrometry, De novo sequencing
Single-pot, Continuous digestion, COVID-19-NAbs
Contact List
Ningshao Xia
contact affiliationState Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, National Institute for Data Science in Health and Medicine, Xiamen University
contact emailnsxia@xmu.edu.cn
lab head
Yueting Xiong
contact affiliationState Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, National Institute for Data Science in Health and Medicine
contact emailytxiong@xmu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI