PXD060500

PXD060500 is an original dataset announced via ProteomeXchange.

Title	A rapid, robust, and refined solution Enhance Accuracy and Throughput in Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies
Description	Elucidating antibody sequences is crucial for understanding antigen binding and advancing therapeutic and research applications. However, current de novo sequencing technology is hindered by high costs, low throughput, missing ion information, and assembly limitations in accuracy and robustness. Here, we developed a refined sequencing solution by integrating Single-Pot Multi-Enzymatic Gradient Digestion (SP-MEGD) with a beam search-based assembler (Fusion) to decipher complete sequences. This solution achieved high accuracy (over 99%) in antibody reconstruction. Biological validation of multiple commercial antibodies with unavailable sequences further supports its effectiveness. Additionally, our solution can discriminate and achieve over 99% accurate sequence coverage of mixtures containing two or three COVID-19 neutralizing antibodies. To our knowledge, this is the first study to enable simultaneous sequencing of multiple antibodies using only bottom-up mass spectrometry, improving throughput without increasing duration or expense. In summary, our method advances antibody research by enhancing the accuracy and efficiency of complex antibody sequencing.
HostingRepository	iProX
AnnounceDate	2025-02-05
AnnouncementXML	Submission_2025-02-04_22:44:06.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yueting Xiong
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	deaminated residue; oxidation; deamidated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-04 22:43:43	ID requested
⏵ 1	2025-02-04 22:44:07	announced

Dataset with its publication pending

submitter keyword: Mass spectrometry, De novo sequencing

Single-pot, Continuous digestion, COVID-19-NAbs

Ningshao Xia
contact affiliation	State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, National Institute for Data Science in Health and Medicine, Xiamen University
contact email	nsxia@xmu.edu.cn
lab head
Yueting Xiong
contact affiliation	State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Public Health, National Institute for Data Science in Health and Medicine
contact email	ytxiong@xmu.edu.cn
dataset submitter

iProX dataset URI