PXD060483 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The ontogeny and inter-individual variability of drug- metabolizing enzymes and transporters in human liver. |
Description | Drug dosing for children is frequently suboptimal when relying on the traditional approach of normalizing doses based on body weight or surface area. Thus, the ontogeny profiling of drug-metabolizing enzymes (DMEs) is important to help develop physiologically-based pharmacokinetic (PBPK) models for predicting safer pediatric dosing. In particular interest are hydrolases are one of the most diverse classes of DMEs and catalyze reactions containing esters, amides, phosphates, etc. in both drugs and prodrugs. Hydrolases such as carboxyl esterases, cathepsins, and arylacetamide deacetylase have been studied for their involvement in drug metabolism. However, a significant number of hydrolases have not yet been appropriately characterized, and as such their influence on drug metabolism remains unknown. Moreover, there is a lack of comprehensive data on the ontogeny and inter-individual variability in expression of these enzymes. Here a characterization of the age-dependent protein abundance of hydrolases in viable primary human hepatocytes isolated from pediatric (n=50) and adult (n=8) donors (Female=28; Male=30) using quantitative global proteomics-based total protein approach was performed. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-12 |
AnnouncementXML | Submission_2025-02-12_08:01:01.597.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bhagwat Prasad |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-04 15:35:00 | ID requested | |
1 | 2025-02-05 12:35:35 | announced | |
⏵ 2 | 2025-02-12 08:01:02 | announced | 2025-02-12: Updated project metadata. |
Publication List
Thakur A, Subash S, Ahire D, Prasad B, Developmental Expression of Drug Transporters and Conjugating Enzymes Involved in Enterohepatic Recycling: Implication for Pediatric Drug Dosing. Clin Pharmacol Ther, 116(6):1615-1626(2024) [pubmed] |
10.1002/cpt.3409; |
Keyword List
submitter keyword: Ontogeny, Human Liver,Hydrolytic drug-metabolizing enzymes |
Contact List
Bhagwat Prasad |
contact affiliation | Principal Investigator, Professor, Department of Pharmaceuitcal Sciences Washington State University, Spokane, WA |
contact email | bhagwat.prasad@wsu.edu |
lab head | |
Bhagwat Prasad |
contact affiliation | Washington State University |
contact email | bhagwat.prasad@wsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060483 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060483
- Label: PRIDE project
- Name: The ontogeny and inter-individual variability of drug- metabolizing enzymes and transporters in human liver.