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PXD060483

PXD060483 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe ontogeny and inter-individual variability of drug- metabolizing enzymes and transporters in human liver.
DescriptionDrug dosing for children is frequently suboptimal when relying on the traditional approach of normalizing doses based on body weight or surface area. Thus, the ontogeny profiling of drug-metabolizing enzymes (DMEs) is important to help develop physiologically-based pharmacokinetic (PBPK) models for predicting safer pediatric dosing. In particular interest are hydrolases are one of the most diverse classes of DMEs and catalyze reactions containing esters, amides, phosphates, etc. in both drugs and prodrugs. Hydrolases such as carboxyl esterases, cathepsins, and arylacetamide deacetylase have been studied for their involvement in drug metabolism. However, a significant number of hydrolases have not yet been appropriately characterized, and as such their influence on drug metabolism remains unknown. Moreover, there is a lack of comprehensive data on the ontogeny and inter-individual variability in expression of these enzymes. Here a characterization of the age-dependent protein abundance of hydrolases in viable primary human hepatocytes isolated from pediatric (n=50) and adult (n=8) donors (Female=28; Male=30) using quantitative global proteomics-based total protein approach was performed.
HostingRepositoryPRIDE
AnnounceDate2025-02-12
AnnouncementXMLSubmission_2025-02-12_08:01:01.597.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBhagwat Prasad
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-04 15:35:00ID requested
12025-02-05 12:35:35announced
22025-02-12 08:01:02announced2025-02-12: Updated project metadata.
Publication List
Thakur A, Subash S, Ahire D, Prasad B, Developmental Expression of Drug Transporters and Conjugating Enzymes Involved in Enterohepatic Recycling: Implication for Pediatric Drug Dosing. Clin Pharmacol Ther, 116(6):1615-1626(2024) [pubmed]
10.1002/cpt.3409;
Keyword List
submitter keyword: Ontogeny, Human Liver,Hydrolytic drug-metabolizing enzymes
Contact List
Bhagwat Prasad
contact affiliationPrincipal Investigator, Professor, Department of Pharmaceuitcal Sciences Washington State University, Spokane, WA
contact emailbhagwat.prasad@wsu.edu
lab head
Bhagwat Prasad
contact affiliationWashington State University
contact emailbhagwat.prasad@wsu.edu
dataset submitter
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Dataset FTP location
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