PXD060483

PXD060483 is an original dataset announced via ProteomeXchange.

Title	The ontogeny and inter-individual variability of drug- metabolizing enzymes and transporters in human liver.
Description	Drug dosing for children is frequently suboptimal when relying on the traditional approach of normalizing doses based on body weight or surface area. Thus, the ontogeny profiling of drug-metabolizing enzymes (DMEs) is important to help develop physiologically-based pharmacokinetic (PBPK) models for predicting safer pediatric dosing. In particular interest are hydrolases are one of the most diverse classes of DMEs and catalyze reactions containing esters, amides, phosphates, etc. in both drugs and prodrugs. Hydrolases such as carboxyl esterases, cathepsins, and arylacetamide deacetylase have been studied for their involvement in drug metabolism. However, a significant number of hydrolases have not yet been appropriately characterized, and as such their influence on drug metabolism remains unknown. Moreover, there is a lack of comprehensive data on the ontogeny and inter-individual variability in expression of these enzymes. Here a characterization of the age-dependent protein abundance of hydrolases in viable primary human hepatocytes isolated from pediatric (n=50) and adult (n=8) donors (Female=28; Male=30) using quantitative global proteomics-based total protein approach was performed.
HostingRepository	PRIDE
AnnounceDate	2025-02-12
AnnouncementXML	Submission_2025-02-12_08:01:01.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bhagwat Prasad
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-04 15:35:00	ID requested
1	2025-02-05 12:35:35	announced
⏵ 2	2025-02-12 08:01:02	announced	2025-02-12: Updated project metadata.

Thakur A, Subash S, Ahire D, Prasad B, Developmental Expression of Drug Transporters and Conjugating Enzymes Involved in Enterohepatic Recycling: Implication for Pediatric Drug Dosing. Clin Pharmacol Ther, 116(6):1615-1626(2024) [pubmed]

10.1002/cpt.3409;

submitter keyword: Ontogeny, Human Liver,Hydrolytic drug-metabolizing enzymes

Bhagwat Prasad
contact affiliation	Principal Investigator, Professor, Department of Pharmaceuitcal Sciences Washington State University, Spokane, WA
contact email	bhagwat.prasad@wsu.edu
lab head
Bhagwat Prasad
contact affiliation	Washington State University
contact email	bhagwat.prasad@wsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060483

PRIDE project URI

• PRIDE
  1. PXD060483
     1. Label: PRIDE project
     2. Name: The ontogeny and inter-individual variability of drug- metabolizing enzymes and transporters in human liver.