PXD060479

PXD060479 is an original dataset announced via ProteomeXchange.

Title	Maize plant infection by Ustilago maydis is regulated by the fungal sulfur metabolism
Description	The infection process is characterized by the simultaneous production of metabolites by both the pathogen and the host. Recently, it has been discovered that molecules generated during sulfur metabolism, mainly hydrogen sulfide (H₂S), play a key role in regulating which organism will prevail during pathogenesis. This phenomenon has been described in bacterial and filamentous fungi infections. Ustilago maydis is a saprophytic fungus that infects maize plants and causes crop losses on a global scale. Although the role of various biomolecules produced by U. maydis during the infection process has been described (ustilagic acid, polyketides, and lipids among others), the function of sulfur-containing compounds, like cysteine and H₂S in this process remains uncharacterized. Here, U. maydis was incubated in a culture medium that promotes H₂S production. The cytoplasmic extract was then obtained, and proteins were extracted and enzymatically digested with trypsin. The resulting peptides were separated using a UPLC NanoAcquity M-Class system. The spectra of these peptides were acquired using MS with electrospray ionization (ESI), employing a data-independent acquisition (DIA) approach and multiplexed high-definition tandem mass spectrometry (HD-MS/MS). Thus, a total of 1,706 proteins were identified and quantified using an internal standard and the intensity of the most abundant peptides, analyzed with Progenesis QI software v4.2.1 and an Ustilago maydis FASTA database (downloaded from UniProt, UP000000561, containing 6,805 protein sequences). Among the identified proteins, 290 were upregulated, while 160 were downregulated. Upon incubation in the aforementioned medium, proteins related to mitochondrial respiration, proteasomal protein degradation, and fatty acid metabolism were upregulated, whereas proteins associated with oxidative stress response, the pentose phosphate pathway (PPP), and translation were downregulated. Additionally, an increase in proteins related to the infectious process, such as endoxylanases and proteinases, was also observed. Thus, this study describes for the first time the role of cysteine metabolism regulation in proteome dynamics in Ustilago maydis.
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-13_10:49:36.787.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilio Espinoza-Simón
SpeciesList	scientific name: Ustilago maydis (Smut fungus); NCBI TaxID: 5270;
ModificationList	iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-04 13:16:33	ID requested
⏵ 1	2025-03-13 10:49:37	announced

Dataset with its publication pending

submitter keyword: mass spectrometry, protein quantification, phytopathogens,Ustilago, label free

Francisco Torres-Quiroz
contact affiliation	Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM)
contact email	ftq@ifc.unam.mx
lab head
Emilio Espinoza-Simón
contact affiliation	Departamento de Bioquímica y Biología Estructural, Intituto de Fisiología Celular. Universidad Nacional Autónoma de México
contact email	eespinoza@ifc.unam.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD060479

PRIDE project URI

• PRIDE
  1. PXD060479
     1. Label: PRIDE project
     2. Name: Maize plant infection by Ustilago maydis is regulated by the fungal sulfur metabolism