PXD060439

PXD060439 is an original dataset announced via ProteomeXchange.

Title	Sheep faeces preservation affects microbiome activity
Description	In vitro systems involving microbial fermentation such as the Hohenheim gas test (HGT) typically need freshly obtained inocula such as rumen fluid or faeces. The objective of this study was to test the possibility of becoming independent of fresh faeces by using preserved faeces. Fresh faeces from sheep (control, C) was compared with seven differently preserved faeces in the HGT by using nine feeds and studying in vitro gas production (GP). Preservation involved freezing at -20°C (FR), shock-freezing with liquid nitrogen (N) and additional freezing at -20°C (FRN), FRN followed by defrosting (FRNdef), shock-freezing with liquid N and freeze-drying (FDN), freeze-drying (FD), freeze-drying and storage for three weeks (FD3W) and six months (FD6M). MA metaproteomics workflow was used to analyse the effects of treatments C, FR, FRN, FD, and FDN on to study changes in microbial community composition and functionactivity.
HostingRepository	PRIDE
AnnounceDate	2026-03-28
AnnouncementXML	Submission_2026-03-27_22:12:23.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Seifert
SpeciesList	scientific name: Ovis aries; NCBI TaxID: NEWT:9940;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-03 11:37:22	ID requested
⏵ 1	2026-03-27 22:12:23	announced

10.1017/ANR.2025.10014;

submitter keyword: metaproteomics, microbiome, faeces

Prof. Jana Seifert
contact affiliation	University of Hohenheim, Functional Microbiology of Livestock, Stuttgart, Germany
contact email	jseifert@uni-hohenheim.de
lab head
Jana Seifert
contact affiliation	University of Hohenheim
contact email	jseifert@uni-hohenheim.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD060439

PRIDE project URI

• PRIDE
  1. PXD060439
     1. Label: PRIDE project
     2. Name: Sheep faeces preservation affects microbiome activity