PXD060406

PXD060406 is an original dataset announced via ProteomeXchange.

Title	Plk1 inhibition delays mitotic entry revealing early changes to the phosphoproteome in cell division
Description	Polo-like kinase 1 (plk1) is a conserved regulator of cell division. During prophase, plk1 phosphorylates direct substrates and is involved in activation of the cyclin-dependent kinase 1 (cdk1). However, the exact functions of plk1 in prophase remain incompletely understood. Here, we inhibited plk1 in synchronous G2 populations of multiple mammalian cell lines. This led to delay or prevention of mitotic entry showing high variability between individual cells. Using a mathematical model, we recapitulate and provide an explanation for this seemingly variable phenotype. We show that plk1-inhibited cells are delayed in a prophase-like state with low cdk1 activity that increases slowly and gradually over hours. These cells display progressively condensing chromosomes, increased microtubule dynamics and reorganization of the actin cortex, while the nuclear envelope remains intact. We then characterize this state by phosphoproteomics, revealing phosphorylation of regulators of chromatin organization and the cytoskeleton consistent with cellular phenotypes. Together, we show that plk1 inhibition stabilizes cells in a prophase-like state with low cdk1 activity displaying a specific set of early mitotic phosphorylations.
HostingRepository	PRIDE
AnnounceDate	2025-03-17
AnnouncementXML	Submission_2025-03-17_05:00:01.910.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olexandr Dybkov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-31 15:35:27	ID requested
⏵ 1	2025-03-17 05:00:02	announced

10.1038/S44318-025-00400-9;

submitter keyword: mitotic entry,cell cycle, mitosis, phosphoproteomics, polo-like kinase 1

Henning Urlaub
contact affiliation	Bioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Germany Bioanalytics, Institute for Clinical Chemistry, University Medical Center Goettingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Olexandr Dybkov
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	odybkov@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD060406

PRIDE project URI

• PRIDE
  1. PXD060406
     1. Label: PRIDE project
     2. Name: Plk1 inhibition delays mitotic entry revealing early changes to the phosphoproteome in cell division