PXD060393

PXD060393 is an original dataset announced via ProteomeXchange.

Title	Nano-omics Enables Plasma-to-Tumour Tissue Integrated Proteomics for Biomarker Discovery in Glioblastoma
Description	The dismal survival of patients with glioblastoma (GB) has only marginally improved over the past two decades, underscoring the urgent need for new technologies to advance early detection and disease monitoring strategies. Despite the promise of 'liquid biopsies’, no blood-based biomarkers have yet been approved for clinical use in GB. In this work, we developed a nanotechnology-enabled workflow to correlate systemic (plasma) and localized (tumour tissue) protein changes associated with GB progression in the brain. We exploited the formation of the biomolecule corona onto blood-circulating liposome nanoparticles (NPs) to uncover longitudinal alterations in the plasma proteome of GL261-bearing female mice across different stages of GB progression. Our results revealed plasma protein changes even at low tumour burden, demonstrating a notable overlap (>30%) between the GB-specific plasma and tumour tissue proteomes, and highlighting a dynamic transition of molecular mechanisms with GB development. Subsequent analysis of matched plasma and surgically resected tumour samples obtained from human high-grade gliomas patients (n=6 male and n=4 female) provided pilot data to demonstrate the applicability of the Nano-omics integrative pipeline in clinical biomarker discovery studies. Furthermore, proof-of-concept cross-species correlation analysis identified a panel of 48 GB biomarker candidates implicated in actin cytoskeleton, focal adhesion, platelet activation, leukocyte migration, amino acid biosynthesis, carbon metabolism, and phagosome pathways. The proposed Nano-omics approach, holds promise for a wide range of conditions affecting the central nervous system, facilitating the discovery of early detection and disease monitoring blood-based biomarkers for subsequent clinical validation.
HostingRepository	PRIDE
AnnounceDate	2025-03-11
AnnouncementXML	Submission_2025-03-11_10:23:42.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinming Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-31 05:37:16	ID requested
⏵ 1	2025-03-11 10:23:43	announced

Dataset with its publication pending

submitter keyword: protein corona, proteomics, neuro-oncology, early detection, liquid-biopsy,brain cancer, biomarkers

Marilena Hadjidemetriou
contact affiliation	NanoOmics Lab, Centre for Nanotechnology in Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK
contact email	marilena.hadjidemetriou@manchester.ac.uk
lab head
Xinming Liu
contact affiliation	University of Manchester
contact email	anita.xliu76@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD060393

PRIDE project URI

• PRIDE
  1. PXD060393
     1. Label: PRIDE project
     2. Name: Nano-omics Enables Plasma-to-Tumour Tissue Integrated Proteomics for Biomarker Discovery in Glioblastoma