PXD060391

PXD060391 is an original dataset announced via ProteomeXchange.

Title	Architecture of the human KICSTOR and GATOR1-KICSTOR complexes
Description	Our understanding of the molecular processes by which cells sense nutrients has seen rapid progress, largely due to new structural andfunctional insights into the mTORC1 pathway. Serving as a key regulator of cellular growth, mTORC1 coordinates various environmental signalssuch as growth factors, nutrients, and energy status, and then converts these signals into responses that promote cell growth and inhibits catabolic processes. The KICSTOR complex is required for lysosomal recruitment of GATOR1, the key mTORC1 repressor. Genetic mutations inthe KICSTOR subunits are linked with severe human neurodevelopmental and epileptic encephalopathy. We combined Cryo-EM and in silicopredictions to assemble the structural models of KICSTOR and GATOR1-KICSTOR supercomplexes. SZT2 exhibits a long crescent-shapedarchitecture, which is built from tandem structured units connected via loops while anchoring other three subunits, including the ITFG2-KPTNheterodimer and C12orf66, to its C-terminal portion. GATOR1 interacts exclusively with the N-terminal domain (NTD) of SZT2. Our workprovides mechanistic insights into the molecular basis of how KICSTOR recruits GATOR1 on the lysosomal surface to regulate the nutrient-dependent signaling of mTORC1.
HostingRepository	PRIDE
AnnounceDate	2025-09-20
AnnouncementXML	Submission_2025-09-20_05:31:20.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Goran Stjepanovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-31 04:34:11	ID requested
⏵ 1	2025-09-20 05:31:20	announced

Dataset with its publication pending

submitter keyword: KICSTOR, x-link, GATOR1

Goran Stjepanovic
contact affiliation	Dr Goran Stjepanovic, Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen, Shenzhen 518172, China.
contact email	goranstjepanovic@cuhk.edu.cn
lab head
Goran Stjepanovic
contact affiliation	CUHKSZ
contact email	goranstjepanovic@cuhk.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD060391

PRIDE project URI

• PRIDE
  1. PXD060391
     1. Label: PRIDE project
     2. Name: Architecture of the human KICSTOR and GATOR1-KICSTOR complexes