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PXD060389

PXD060389 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic characterisation of Aspergillus fumigatus – host interactions using the ex-vivo pig lung model (EVPL)
DescriptionAspergillus fumigatus is an environmental saprophyte and an opportunistic fungal pathogen of the human airway. A. fumigatus can cause a variety of chronic infections, typically in the context of pre-existing lung damage. Animal models of infection are costly and often cannot recapitulate phenotypes observed in patients. The ex-vivo pig lung (EVPL) model was developed for conducting bacterial infection studies as pigs share 90% immunological homology to humans and display many anatomical similarities. EVPL also retains resident immune cells and richer cellular complexity compared to in-vitro models, in addition to a microbiome. Proteomic analysis of A. fumigatus infected alveolar tissue enabled the tracking of molecular changes to the pathogen and the host during the establishment of fungal infection. Analysis identified the metabolism and development of A. fumigatus on the EVPL sections and indicated a shift from initial carbon metabolism to protein metabolism with an emphasis on amino acid metabolism and biosynthesis. The tissue remained responsive to the pathogen with proteins increased in abundance associated with innate immune recruitment at 24 hours, while proteins associated with neutrophil degranulation were decreased in abundance. At 96 hours the infected tissue demonstrated enhanced expression of fibrotic markers relative to the uninfected control. These similarities in response validate the use of this model and contributes supporting evidence that these patterns are of clinical importance during A. fumigatus colonisation and infection of pulmonary tissue.
HostingRepositoryPRIDE
AnnounceDate2025-05-20
AnnouncementXMLSubmission_2025-05-20_03:54:13.401.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAaron Curtis
SpeciesList scientific name: Sus scrofa scrofa; NCBI TaxID: 415978; scientific name: Aspergillus fumigatus var. fumigatus; NCBI TaxID: 41122;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-31 02:26:03ID requested
12025-05-20 03:54:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Aspergillus, Infection, ex vivo, Porcine, Proteomics
Contact List
Kevin Kavanagh
contact affiliationMaynooth University
contact emailKevin.kavanagh@mu.ie
lab head
Aaron Curtis
contact affiliationMaynooth Univeristy
contact emailaaron.curtis.2018@mumail.ie
dataset submitter
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Dataset FTP location
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