PXD060381

PXD060381 is an original dataset announced via ProteomeXchange.

Title	CRISPR/dCAS9 genomic engineering for boosting the therapeutic potential of Extracellular Vesicles
Description	This study aimed to enhance the therapeutic effect of MSC-derived extracellular vesicles (EVs) by overexpressing the TSG-6 gene using CRISPR activation and evaluate the biological activity of these modified EVs on human intervertebral disc (IVD) cells in vitro. n immortalized human MSC line was transduced with a CRISPR activation system targeting TSG-6. MSC-EVs were collected via ultracentrifugation, and their particle size/number was analyzed. Gene and protein expression were assessed to evaluate transduction efficiency. EV proteomics was analyzed by mass spectrometry. Human IVD cells were isolated from patients undergoing spinal surgery, pre-stimulated with IL-1β, and treated with MSC-EVs.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:06:12.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iker Martinez Zalbidea
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-30 14:06:02	ID requested
⏵ 1	2025-05-07 07:06:13	announced

10.1007/s12195-025-00843-4;

Martinez-Zalbidea I, Wagner G, Bergendahl N, Mesfin A, Puvanesarajah V, Hitzl W, Schulze S, Wuertz-Kozak K, CRISPR-dCas9 Activation of TSG-6 in MSCs Modulates the Cargo of MSC-Derived Extracellular Vesicles and Attenuates Inflammatory Responses in Human Intervertebral Disc Cells In Vitro. Cell Mol Bioeng, 18(1):83-98(2025) [pubmed]

submitter keyword: Human, MSCs, Extracellular Vesicles, CRISPR

Karin Wuertz-Kozak
contact affiliation	Kate Gleason Endowed Full Professor Biomedical Engineering Rochester Institute of Technology Rochester, New York. USA.
contact email	kwbme@rit.edu
lab head
Iker Martinez Zalbidea
contact affiliation	Rochester Institute of Technology
contact email	im5865@rit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060381

PRIDE project URI

• PRIDE
  1. PXD060381
     1. Label: PRIDE project
     2. Name: CRISPR/dCAS9 genomic engineering for boosting the therapeutic potential of Extracellular Vesicles