PXD060378 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | High-Sensitive Spatial Proteomics for Pancreatic Cancer Progression Analysis |
| Description | Pancreatic cancer remains as one of the most challenging malignancies to diagnose and treat due to its silent progression and limited early diagnostic options. The intraductal papillary mucinous neoplasm (IPMN) provides an early-stage lesion for studying pancreatic cancer progression. However, proteomic analyses of IPMN and its comparison with pancreatic ductal adenocarcinoma (PDAC) and normal duct (ND) have been limited due to their low cellularity. In this study, we present an optimized spatial tissue proteomics workflow, termed SP-Max (Spatial Proteomics Optimized for Maximum Sensitivity and Reproducibility in Minimal Sample), designed to maximize protein recovery and quantification from limited laser micro dissected (LMD) samples. Our workflow enabled the identification of more than 6,000 proteins and the quantification of over 5,200 protein groups from FFPE tissue contours of actual pancreatic cancer patients. Comparative analyses across ND, IPMN, and PDAC revealed critical molecular differences in protein pathways and potential prognostic markers. SP-Max provides a systematic, reproducible approach that significantly enhances our ability to study precancerous lesions and cancer progression in pancreatic tissues at unprecedented resolution. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-23_06:54:10.735.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jongmin Woo |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF HT; Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-30 13:09:59 | ID requested | |
| ⏵ 1 | 2026-02-23 06:54:11 | announced | |
Publication List
Keyword List
| submitter keyword: Pancreatic cancer,FFPE tissue spatial proteomics, IPMN to PDAC progression, High-sensitivity mass spectrometry |
Contact List
| Hui Zhang |
|---|
| contact affiliation | Johns Hopkins University |
| contact email | huizhang@jhu.edu |
| lab head | |
| Jongmin Woo |
|---|
| contact affiliation | University of Maryland Baltimore |
| contact email | jongminw@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060378 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060378
- Label: PRIDE project
- Name: High-Sensitive Spatial Proteomics for Pancreatic Cancer Progression Analysis
