PXD060374

PXD060374 is an original dataset announced via ProteomeXchange.

Title	Exploring the high light stress response of a Haematococcus pluvialis (Chlorophyceae) mutant with a higher astaxanthin content via proteomic and phosphoproteomic analysis reveals altered chlorophyll biosynthesis, carbon partitioning, and astaxanthin biosynthesis and trafficking
Description	While the green microalga, Haematococcus pluvialis, is the best natural producer of astaxanthin, efforts to grow it for astaxanthin production are still limited by its low biomass productivity which subsequently results in lower astaxanthin productivity. Despite new methods of direct strain modification to improve growth and astaxanthin production, the limited understanding of how the astaxanthin biosynthesis pathway is regulated has been a significant roadblock to strain improvement. This work aims to address this knowledge gap by exploring the stress response of H. pluvialis during the transition from heterotrophy to high light conditions. A physical radiation (heavy-ion beam) mutagenesis approach was applied to generate H. pluvialis mutants with a higher cell division rate under heterotrophic conditions. The mutant JWHIB 27-38 that grew 25% faster than the wild type was identified. Mutant JWHIB 27-38 also achieved a 69.61% higher lipid content per cell and 86.17% higher astaxanthin per cell than the wild type under high light stress conditions. A proteomics and phosphoproteomics analysis was conducted with JWHIB 27-38 and the wild type at 0, 48, and 72 h of high light stress to elucidate the mechanisms underlying these phenotypes. Maintained upregulation of chlorophyll biosynthesis pathway proteins and phosphoproteins in mutant JWHIB 27-38 during high light stress, such as glutamate-1-semialdehyde 2,1-aminomutase (GSAM) and coproporphyrinogen oxidase (CPOX), may support continued chlorophyll biosynthesis which can allow cells to adapt to high light conditions. Upregulation of fatty acid biosynthetic proteins such as biotin carboxyl carrier protein (BCCP) and 3-ketoacyl-CoA synthase (KCS) in mutant JWHIB 27-38 suggests that carbon is favored toward lipid accumulation. Increased lipid content, along with upregulation of the key astaxanthin biosynthesis protein, phytoene synthase (PSY), may enable the higher astaxanthin content in mutant JWHIB 27-38. Additionally, upregulation of a putative astaxanthin-trafficking protein, AstaP, likely enables the mutant to traffic more astaxanthin to the cell periphery as part of its stress response. These and other significantly differentially expressed proteins may provide promising targets for future strain engineering work to improve astaxanthin productivity.
HostingRepository	PRIDE
AnnounceDate	2026-01-24
AnnouncementXML	Submission_2026-01-24_14:56:45.126.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060374
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kyarii Ramarui
SpeciesList	scientific name: Haematococcus lacustris; NCBI TaxID: NEWT:44745;
ModificationList	phosphorylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-30 10:40:30	ID requested
⏵ 1	2026-01-24 14:56:45	announced

10.6019/PXD060374;

10.1016/J.ALGAL.2026.104546;

submitter keyword: lipid, proteomics, post-translational modification,High light stress, astaxanthin, phosphoproteomics, chlorophyll biosynthesis, cell cycle control

Yantao Li
contact affiliation	Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science and University of Maryland Baltimore County, 701 E Pratt Street, Baltimore MD, 21202, United States.
contact email	yantao@umces.edu
lab head
Kyarii Ramarui
contact affiliation	University of Maryland Center for Environmental Science -- Institute of Marine and Environmental Technology
contact email	kramarui@umces.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060374
     1. Label: PRIDE project
     2. Name: Exploring the high light stress response of a Haematococcus pluvialis (Chlorophyceae) mutant with a higher astaxanthin content via proteomic and phosphoproteomic analysis reveals altered chlorophyll biosynthesis, carbon partitioning, and astaxanthin biosynthesis and trafficking