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PXD060372

PXD060372 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome-wide profiling of olaparib interactors using a biotinylated photoaffinity probe
DescriptionOlaparib is a widely used PARP inhibitor for the treatment of BRCA-mutated cancers. To comprehensively understand the drug's clinical impact, measuring its interactions with intended on- and off-targets is crucial. In this study, olaparib's on- and off-targets were profiled using photoaffinity labeling, a powerful, proteome-wide method for studying the direct interactions between a drug and its protein targets. A novel photoaffinity probe was designed and used in a proteomic screening to discover novel targets of olaparib in the human proteome. The probe, incorporating a pre-installed biotin group, bypasses the limitations of using a copper(I)-catalyzed click reaction in cell lysates for reporter group conjugation and revealed a broad range of olaparib interactors, including previously unreported proteins, in a quantitative mass spectrometry-based proteomic screening using HeLa whole cell lysate. The study contributes to our current understanding of the pharmacology of olaparib and provides a valuable tool for elucidating drug interactors within cell lysates, potentially guiding the development of more targeted therapeutics with fewer off-targets.
HostingRepositoryPRIDE
AnnounceDate2025-02-04
AnnouncementXMLSubmission_2025-02-04_03:38:43.380.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSuzanne Weijers
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-30 08:52:43ID requested
12025-02-04 03:38:43announced
Publication List
10.1002/CBIC.202400882;
Keyword List
submitter keyword: PARP inhibitors, Photoaffinity labeling,Chemical proteomics, Olaparib
Contact List
Michiel Vermeulen
contact affiliation1. Department of Molecular Biology, RIMLS, Radboud University 2. Division of Molecular Genetics, Netherlands Cancer Institute
contact emailmichiel.vermeulen@ru.nl
lab head
Suzanne Weijers
contact affiliationNetherlands Cancer Institute
contact emails.weijers@nki.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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