PXD060372 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide profiling of olaparib interactors using a biotinylated photoaffinity probe |
Description | Olaparib is a widely used PARP inhibitor for the treatment of BRCA-mutated cancers. To comprehensively understand the drug's clinical impact, measuring its interactions with intended on- and off-targets is crucial. In this study, olaparib's on- and off-targets were profiled using photoaffinity labeling, a powerful, proteome-wide method for studying the direct interactions between a drug and its protein targets. A novel photoaffinity probe was designed and used in a proteomic screening to discover novel targets of olaparib in the human proteome. The probe, incorporating a pre-installed biotin group, bypasses the limitations of using a copper(I)-catalyzed click reaction in cell lysates for reporter group conjugation and revealed a broad range of olaparib interactors, including previously unreported proteins, in a quantitative mass spectrometry-based proteomic screening using HeLa whole cell lysate. The study contributes to our current understanding of the pharmacology of olaparib and provides a valuable tool for elucidating drug interactors within cell lysates, potentially guiding the development of more targeted therapeutics with fewer off-targets. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-04 |
AnnouncementXML | Submission_2025-02-04_03:38:43.380.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Suzanne Weijers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Astral |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-01-30 08:52:43 | ID requested | |
⏵ 1 | 2025-02-04 03:38:43 | announced | |
Publication List
Keyword List
submitter keyword: PARP inhibitors, Photoaffinity labeling,Chemical proteomics, Olaparib |
Contact List
Michiel Vermeulen |
contact affiliation | 1. Department of Molecular Biology, RIMLS, Radboud University 2. Division of Molecular Genetics, Netherlands Cancer Institute |
contact email | michiel.vermeulen@ru.nl |
lab head | |
Suzanne Weijers |
contact affiliation | Netherlands Cancer Institute |
contact email | s.weijers@nki.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060372 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060372
- Label: PRIDE project
- Name: Proteome-wide profiling of olaparib interactors using a biotinylated photoaffinity probe