PXD060372

PXD060372 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide profiling of olaparib interactors using a biotinylated photoaffinity probe
Description	Olaparib is a widely used PARP inhibitor for the treatment of BRCA-mutated cancers. To comprehensively understand the drug's clinical impact, measuring its interactions with intended on- and off-targets is crucial. In this study, olaparib's on- and off-targets were profiled using photoaffinity labeling, a powerful, proteome-wide method for studying the direct interactions between a drug and its protein targets. A novel photoaffinity probe was designed and used in a proteomic screening to discover novel targets of olaparib in the human proteome. The probe, incorporating a pre-installed biotin group, bypasses the limitations of using a copper(I)-catalyzed click reaction in cell lysates for reporter group conjugation and revealed a broad range of olaparib interactors, including previously unreported proteins, in a quantitative mass spectrometry-based proteomic screening using HeLa whole cell lysate. The study contributes to our current understanding of the pharmacology of olaparib and provides a valuable tool for elucidating drug interactors within cell lysates, potentially guiding the development of more targeted therapeutics with fewer off-targets.
HostingRepository	PRIDE
AnnounceDate	2025-02-04
AnnouncementXML	Submission_2025-02-04_03:38:43.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suzanne Weijers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-30 08:52:43	ID requested
⏵ 1	2025-02-04 03:38:43	announced

10.1002/CBIC.202400882;

submitter keyword: PARP inhibitors, Photoaffinity labeling,Chemical proteomics, Olaparib

Michiel Vermeulen
contact affiliation	1. Department of Molecular Biology, RIMLS, Radboud University 2. Division of Molecular Genetics, Netherlands Cancer Institute
contact email	michiel.vermeulen@ru.nl
lab head
Suzanne Weijers
contact affiliation	Netherlands Cancer Institute
contact email	s.weijers@nki.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060372

PRIDE project URI

• PRIDE
  1. PXD060372
     1. Label: PRIDE project
     2. Name: Proteome-wide profiling of olaparib interactors using a biotinylated photoaffinity probe