PXD060363

PXD060363 is an original dataset announced via ProteomeXchange.

Title	Identification of global proteome changes upon rapalog-induced tethering of WIPI2 to the mitochondrial outer membrane in wild-type or NIX/BNIP3 DKO HeLa cells
Description	We aimed to identify proteins that would be turned over upon artificial tethering of WIPI2 to the mitochondrial surface upon rapalog treatment, using the FIS1-FRB plus FKBP-GFP-WIPI2 tethering system. We had previously observed that this induces mitophagy based on flow cytometry analysis of the mt-mKeima probe. Here, we aimed to identify which proteins are degraded upon 24 h Rapalog treatment and verified whether there was an enrichment of mitochondrial proteins being selectively turned over by mitophagy under these conditions in wild-type and NIX/BNIP3 double-knockout HeLa cells.
HostingRepository	PRIDE
AnnounceDate	2025-01-30
AnnouncementXML	Submission_2025-01-30_09:24:44.763.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-30 05:30:50	ID requested
⏵ 1	2025-01-30 09:24:45	announced

Dataset with its publication pending

submitter keyword: mitophagy, WIPI2, BNIP3, hypoxia, mitochondria,NIX

Sascha Martens
contact affiliation	Max Perutz Labs, Vienna BioCenter, University of Vienna Dr. Bohr-Gasse 9/5, 1030 Vienna, Austria
contact email	Sascha.martens@univie.ac.at
lab head
Markus Hartl
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact email	proteomics@maxperutzlabs.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD060363

PRIDE project URI

• PRIDE
  1. PXD060363
     1. Label: PRIDE project
     2. Name: Identification of global proteome changes upon rapalog-induced tethering of WIPI2 to the mitochondrial outer membrane in wild-type or NIX/BNIP3 DKO HeLa cells