PXD060340

PXD060340 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Rat Kir4.1 interactome with the particular purpose to identify 10 kDa copurified proteins using LC-MSMS
Description	The project aims to determine the interactome of full-length Kir4.1 overexpressed in HEK293 stable cells. The overexpressed proteins were purified, and the purified samples, along with three bands from a 1D SDS-PAGE gel, were analyzed by LC-MSMS after trypsin digestion. The primary goal was to identify the highly populated 10 kDa proteins, which were likely direct binders of Kir4.1 proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:58:22.589.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060340
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sun-Joo Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-29 15:13:34	ID requested
⏵ 1	2025-05-07 06:58:23	announced

Publication List

10.6019/PXD060340;
Lee SJ, Gao J, Thompson E, Mount J, Nichols CG, Dynein light chains 1 and 2 are auxiliary proteins of pH-sensitive Kir4.1 channels. J Biol Chem, 301(4):108393(2025) [pubmed]
10.1016/j.jbc.2025.108393;

10.6019/PXD060340;

Lee SJ, Gao J, Thompson E, Mount J, Nichols CG, Dynein light chains 1 and 2 are auxiliary proteins of pH-sensitive Kir4.1 channels. J Biol Chem, 301(4):108393(2025) [pubmed]

10.1016/j.jbc.2025.108393;

Keyword List

submitter keyword: LC-MSMS, HEK293 stable cells,Full length rat Kir4.1, LC8 copurification

submitter keyword: LC-MSMS, HEK293 stable cells,Full length rat Kir4.1, LC8 copurification

Contact List

Colin G. Nichols
contact affiliation	Washington University in St. Louis, School of Medicine, Department of Cell Biology and Physiology,Center for Investigation of Membrane Excitability Diseases
contact email	cnichols@wustl.edu
lab head
Sun-Joo Lee
contact affiliation	Washington University in St. Louis
contact email	sunjoo.lee@wustl.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060340
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060340

PRIDE project URI

Repository Record List

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