PXD060340
PXD060340 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rat Kir4.1 interactome with the particular purpose to identify 10 kDa copurified proteins using LC-MSMS |
Description | The project aims to determine the interactome of full-length Kir4.1 overexpressed in HEK293 stable cells. The overexpressed proteins were purified, and the purified samples, along with three bands from a 1D SDS-PAGE gel, were analyzed by LC-MSMS after trypsin digestion. The primary goal was to identify the highly populated 10 kDa proteins, which were likely direct binders of Kir4.1 proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_06:58:22.589.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD060340 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sun-Joo Lee |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-01-29 15:13:34 | ID requested | |
⏵ 1 | 2025-05-07 06:58:23 | announced |
Publication List
10.6019/PXD060340; |
Lee SJ, Gao J, Thompson E, Mount J, Nichols CG, Dynein light chains 1 and 2 are auxiliary proteins of pH-sensitive Kir4.1 channels. J Biol Chem, 301(4):108393(2025) [pubmed] |
10.1016/j.jbc.2025.108393; |
Keyword List
submitter keyword: LC-MSMS, HEK293 stable cells,Full length rat Kir4.1, LC8 copurification |
Contact List
Colin G. Nichols | |
---|---|
contact affiliation | Washington University in St. Louis, School of Medicine, Department of Cell Biology and Physiology,Center for Investigation of Membrane Excitability Diseases |
contact email | cnichols@wustl.edu |
lab head | |
Sun-Joo Lee | |
contact affiliation | Washington University in St. Louis |
contact email | sunjoo.lee@wustl.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060340 |
PRIDE project URI |
Repository Record List
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