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PXD060340

PXD060340 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRat Kir4.1 interactome with the particular purpose to identify 10 kDa copurified proteins using LC-MSMS
DescriptionThe project aims to determine the interactome of full-length Kir4.1 overexpressed in HEK293 stable cells. The overexpressed proteins were purified, and the purified samples, along with three bands from a 1D SDS-PAGE gel, were analyzed by LC-MSMS after trypsin digestion. The primary goal was to identify the highly populated 10 kDa proteins, which were likely direct binders of Kir4.1 proteins.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_06:58:22.589.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD060340
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSun-Joo Lee
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-29 15:13:34ID requested
12025-05-07 06:58:23announced
Publication List
10.6019/PXD060340;
Lee SJ, Gao J, Thompson E, Mount J, Nichols CG, Dynein light chains 1 and 2 are auxiliary proteins of pH-sensitive Kir4.1 channels. J Biol Chem, 301(4):108393(2025) [pubmed]
10.1016/j.jbc.2025.108393;
Keyword List
submitter keyword: LC-MSMS, HEK293 stable cells,Full length rat Kir4.1, LC8 copurification
Contact List
Colin G. Nichols
contact affiliationWashington University in St. Louis, School of Medicine, Department of Cell Biology and Physiology,Center for Investigation of Membrane Excitability Diseases
contact emailcnichols@wustl.edu
lab head
Sun-Joo Lee
contact affiliationWashington University in St. Louis
contact emailsunjoo.lee@wustl.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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