PXD060317

PXD060317 is an original dataset announced via ProteomeXchange.

Title	ARMC1 shuttling regulates cellular mitochondrial distribution - whole cell proteome
Description	Mitochondria are platforms for important processes including mitochondrial fusion and fission, protein and lipid transport, cytoskeletal trafficking, and the activation of mitophagy, apoptosis, and innate immune responses. How cells regulate the assembly of protein complexes at the mitochondrial surface to control these dynamic functions is incompletely understood. Here, we show that ARMC1 shuttles between the cytosol and distinct mitochondrial complexes. The assembly of ARMC1 with Miro, MTFR, and TMEM11 links together mitochondrial trafficking and shaping activities and is essential for MTFR stability. In another complex, ARMC1 engages, via MTCH2, DNAJC11, which facilitates ARMC1 recycling back into the cytosol. Disrupting the balance of ARMC1 between these complexes leads to aberrant cellular mitochondrial distributions. Thus, the stability and function of the Miro-MTFR complex is regulated by ARMC1, whose mito-cytoplasmic shuttling via DNAJC11 tunes mitochondrial dynamics. Our findings provide a paradigm for protein complex regulation through the spatial control of a linchpin subunit.
HostingRepository	PRIDE
AnnounceDate	2025-03-19
AnnouncementXML	Submission_2025-03-19_12:30:45.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	João Coelho
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-29 06:40:08	ID requested
⏵ 1	2025-03-19 12:30:46	announced

10.1126/SCIADV.ADU5091;

submitter keyword: whole cell proteome,TMT-proteomics

Sichen Shao
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston, MA, USA
contact email	sichen_shao@hms.harvard.edu
lab head
João Coelho
contact affiliation	Harvard Medical School, Cell Biology Department
contact email	joao_coelho@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060317
     1. Label: PRIDE project
     2. Name: ARMC1 shuttling regulates cellular mitochondrial distribution - whole cell proteome