PXD060313

PXD060313 is an original dataset announced via ProteomeXchange.

Title	Retron Eco2 breaks tRNAs for anti-phage defense
Description	Retrons mediate bacterial anti-phage defense by reverse transcribing non-coding RNA into multicopy single-stranded DNA (msDNA). Our study reveals the unique trimeric nucleoprotein structure of Eco2, which solely relies on a single protein for defense, unlike most other retrons. This structure supports an msDNA-dependent regulation of the Eco2's reverse-transcriptase and TOPRIM/RNase H (TR) fusion protein. We also show that Eco2's broad defense against various phages is triggered by a phage-encoded endonuclease that degrades the msDNA. msDNA decay in turn activates the TR domain for tRNA cleavage, resulting in shutdown of gene expression for abortive infection. Our findings not only advance the understanding of Eco2’s biogenesis and defense mechanism but also provide a structural basis for engineering of this system.
HostingRepository	PRIDE
AnnounceDate	2026-02-03
AnnouncementXML	Submission_2026-02-03_01:18:39.127.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wieland Steinchen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-29 05:16:25	ID requested
⏵ 1	2026-02-03 01:18:39	announced

Dataset with its publication pending

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: Retron, cryo-EM, HDX-MS, anti-phage defense

Gert Bange
contact affiliation	Center for Synthetic Microbiology (SYNMIKRO) & Department of Chemistry, Philipps-University Marburg, Marburg, Germany
contact email	gert.bange@synmikro.uni-marburg.de
lab head
Wieland Steinchen
contact affiliation	Philipps-University Marburg, Department of Chemistry & SYNMIKRO, Karl-von-Frisch-Straße 14, 35043 Marburg, GERMANY
contact email	wieland.steinchen@synmikro.uni-marburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD060313

PRIDE project URI

• PRIDE
  1. PXD060313
     1. Label: PRIDE project
     2. Name: Retron Eco2 breaks tRNAs for anti-phage defense