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PXD060304

PXD060304 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutomated enrichment and co-extraction pipeline for efficient analysis of urinary extracellular vesicle protein and RNA
DescriptionExtracellular vesicles (EVs) cargoes have great potential as promising biomarkers for disease diagnosis. However, traditional methods for EV enrichment and cargo extraction are inefficient, limiting the accessibility of valuable biological information. Here, we present a bead-based pipeline, designed to efficiently enrich EVs from urine while simultaneously extracting both EV protein and RNA. Compared to traditional methods involving ultracentrifugation (UC) for EV isolation, acetone precipita-tion for protein extraction, and TRIzol for RNA extraction, the new pipeline achieved over a 5-fold improvement in EV protein and RNA yield. Additionally, it reduced the whole duration from approximately 2 days to just 1 h. When applied to the non-invasive diagnosis of prostate cancer (PCa), the pipeline demonstrated excellent diagnostic performance, with a panel of EV protein and RNA markers achieving an AUC of 0.82. This efficient and streamlined pipeline provides a robust tool for com-prehensive EV cargo analysis and holds great potential for advancing non-invasive diagnostics in clinical settings.
HostingRepositoryPRIDE
AnnounceDate2025-04-12
AnnouncementXMLSubmission_2025-04-11_16:02:51.659.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYufeng Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-28 22:23:42ID requested
12025-04-11 16:02:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Protein, Co-extraction,Extracellular Vesicles, RNA, Automation, Urine
Contact List
Ying Zhang
contact affiliationDepartment of Chemistry and Institutes of Biomedical Sciences, Fudan University
contact emailying@fudan.edu.cn
lab head
Yufeng Liu
contact affiliationSoutheast University
contact emailliuyufeng@seu.edu.cn
dataset submitter
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Dataset FTP location
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PRIDE project URI
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