PXD060292

PXD060292 is an original dataset announced via ProteomeXchange.

Title	Platelets accelerate endoplasmic reticulum stress and promote hepatic steatosis in mice
Description	Background: Metabolic dysfunction-associated steatotic liver disease (MASLD), the most common chronic liver disease, is characterized by endoplasmic reticulum (ER) stress, which impairs hepatic lipoprotein metabolism. Platelets, which can modulate cellular responses to ER stress, accumulate in the liver during MASLD and contribute to disease development by interacting with liver-resident (immune) cells. Since the role of platelets in modulating hepatic ER stress in vivo is completely unknown, this study aims to investigate their effects on hepatic ER stress and steatosis. Methods/Results: Using C57BL/6 mice, we demonstrate that hepatic ER stress leads to the accumulation and activation of platelets. Depletion of platelets decreased ER stress-induced liver steatosis, indicated by reduced liver triglyceride levels. Mechanistically, we found that platelet depletion ameliorated the initial ER stress response and increased the influx of neutrophils into the liver. Inhibiting the platelet-specific receptor glycoprotein 1bα (GPIbα) partly mimicked the effects of platelet depletion, leading to impaired hepatic platelet recruitment and reduced triglyceride accumulation. Conclusion: This study demonstrates a new role of platelets in hepatic ER stress and the concomitant effect on hepatic steatosis.
HostingRepository	PRIDE
AnnounceDate	2026-03-26
AnnouncementXML	Submission_2026-03-26_10:58:44.033.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrich Stelzl
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-28 13:10:30	ID requested
⏵ 1	2026-03-26 10:58:44	announced

10.1016/J.JHEPR.2026.101767;

submitter keyword: MASLD,Blood platelets, liver, ER stress

Ulrich Stelzl
contact affiliation	University of Graz, Pharmaceutical Sciences, Pharmaceutical Chemistry
contact email	ulrich.stelzl@uni-graz.at
lab head
Ulrich Stelzl
contact affiliation	University of Graz
contact email	ulrich.stelzl@uni-graz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD060292

PRIDE project URI

• PRIDE
  1. PXD060292
     1. Label: PRIDE project
     2. Name: Platelets accelerate endoplasmic reticulum stress and promote hepatic steatosis in mice