PXD060277

PXD060277 is an original dataset announced via ProteomeXchange.

Title	Transcriptomic and proteomic analysis of quiescent epimastigotes as a resource for investigating the persister phenotype in Trypanosoma cruzi.
Description	The “persister” phenotype is often associated with treatment failure. Our knowledge of parasite biology, in particular the mechanistic processes associated with persistence phenotype is limited The ability to isolate homogenous populations of dormant or slowly replicating parasites vs non-perturbed parasites is the limiting step in defining these phenotypes In this context, the project implemented: The use of “fluorescent timer” proteins and live cell sorting as a method for isolating populations of non-perturbed slow-growing or dormant parasites Proteomics and transcriptomics methods, including a common sample collection/sample preparation procedure, from the parasites coming from the sorting to assess the mechanistic processes associated with parasite persistence The aim is the descritpion and to identify discriminant markers/pathways associated with parasite persistence
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-15_07:21:01.059.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Josephine Abi Ghanem
SpeciesList	scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-28 05:30:01	ID requested
⏵ 1	2025-09-15 07:21:01	announced

Dataset with its publication pending

submitter keyword: amastigotes, quiescence, trypomastigotes, epimastigotes,Trypanosoma cruzi

Josephine Abi Ghanem
contact affiliation	Team Lead Computational Biology, OMICS Unit, BIOASTER
contact email	josephineabighanem@gmail.com
lab head
Josephine Abi Ghanem
contact affiliation	BIOASTER
contact email	josephineabighanem@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD060277

PRIDE project URI

• PRIDE
  1. PXD060277
     1. Label: PRIDE project
     2. Name: Transcriptomic and proteomic analysis of quiescent epimastigotes as a resource for investigating the persister phenotype in Trypanosoma cruzi.