PXD060264

PXD060264 is an original dataset announced via ProteomeXchange.

Title	Small molecules reveal differential shifts in stability and protein binding for G-quadruplex RNA
Description	The potential of therapeutically targeting RNA with small molecules continues to grow yet progress is hindered by difficulties in determining specific mechanisms of action, including impacts on RNA-protein binding. RNA G-quadruplexes (rGQs) are a particularly promising target due to their range of biological functions, structural stability, and hydrophobic surfaces, which promote small molecule and protein interactions alike. Challenges arise due to 1) the low structural diversity among rGQs, thereby limiting binding selectivity, and 2) a lack of knowledge regarding how small molecules can manipulate rGQ-protein binding on a global scale. We first leveraged a small molecule library privileged for RNA tertiary structures that displayed differential binding to rGQs based on loop length, consistent with computational predictions for DNA GQs. We next utilized an RT-qPCR-based assay to measure stability against enzymatic readthrough, expected to be a common mechanism in rGQ function. We discovered small molecules with significant, bidirectional impacts on rGQ stability, even within the same scaffold. Using Stability of Proteins from Rates of Oxidation (SPROX), a stability-based proteomics method, we then eluci-dated proteome level impacts of both stabilizing and destabilizing rGQ-targeting molecules on rGQ-protein interactions. This technique revealed small molecule-induced impacts on a unique subset of rGQ-binding proteins, along with proteins that exhibited differential changes based on the identity of the small molecule. The domain and peptide-level insights resulting from SPROX allow for the generation of specific hypotheses for both rGQ function and small molecule modulation thereof. Taken altogether, this methodology helps bridge the gap between small molecule-RNA targeting and RNA-protein interac-tions, providing insight into how small molecules can influence protein binding partners through modulation of target RNA structures.
HostingRepository	PRIDE
AnnounceDate	2026-06-23
AnnouncementXML	Submission_2026-06-23_11:48:00.267.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Labbe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	methylthiolated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 12:46:32	ID requested
⏵ 1	2026-06-23 11:48:00	announced

Dataset with its publication pending

submitter keyword: proteomics,RNA, SPROX

Michael C. Fitzgerald
contact affiliation	Chemistry, Duke University, USA
contact email	michael.c.fitzgerald@duke.edu
lab head
Natalie Labbe
contact affiliation	Duke University Department of Chemistry
contact email	natalie.labbe@duke.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060264
     1. Label: PRIDE project
     2. Name: Small molecules reveal differential shifts in stability and protein binding for G-quadruplex RNA