PXD060246

PXD060246 is an original dataset announced via ProteomeXchange.

Title	Molecular analysis of brain tumour models
Description	Pilocytic astrocytomas and other paediatric low-grade gliomas (pLGGs) are often considered single-pathway diseases due to the predominance of MAPK/ERK pathway alterations observed in these patients. However, emerging evidence suggests that the MAPK/ERK interacts within a complex signalling network that drives the distinct phenotypes of these tumours. To investigate how these signalling pathways intersect across multiple molecular levels, we have conducted multi-omics analysis of a set of in vitro pLGG models developed by Dr Pratiti Bandopadyay and colleagues at the Dana Farber Cancer Institute, USA, using immortalised H9 neural stem cells engineered to carry the critical BRAF or FGFR driver alterations. The proteomic and phosphoproteomic data are included here.
HostingRepository	PRIDE
AnnounceDate	2025-05-14
AnnouncementXML	Submission_2025-05-14_01:23:54.545.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD060246
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Denise Sheer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 04:59:41	ID requested
⏵ 1	2025-05-14 01:23:54	announced

10.6019/PXD060246;

submitter keyword: KIAA1549, Label-free Proteomics, MAPK,BRAF, pLGG, phosphoproteomics, FGFR, LC-MS/MS

Professor Pedro R Cutillas
contact affiliation	BCI Mass spectrometry Facility 1.02 & 1.03 1st floor Barts Cancer Institute Queen Mary, University of London John Vane Science Centre, Charterhouse Square, London EC1M 6BQ
contact email	p.cutillas@qmul.ac.uk
lab head
Denise Sheer
contact affiliation	Blizard Institute, Queen Mary University of London, London, UK, E1 2AT
contact email	d.sheer@qmul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060246

PRIDE project URI

• PRIDE
  1. PXD060246
     1. Label: PRIDE project
     2. Name: Molecular analysis of brain tumour models