PXD060235
PXD060235 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | PEG6000 precipitation and albumin depletion for extended plasma proteomics analysis |
Description | The Mass spectrometry (MS)-based blood plasma or serum proteome analysis is limited by interference from albumin, immunoglobins and other commonly abundant proteins. We have found that polyethylene glycol (PEG) can efficiently precipitate immunoglobins while leave albumin largely in the supernatant. By PEG precipitation followed by albumin depletion, an increased number of proteins and N-glycoproteins can be identified by LC-MS/MS from the plasma. In-depth LC-LC-MS/MS proteomic analyses of the plasma and its four fractionated samples (10% and further 20% PEG-precipitated pellets and their supernatants with albumin depletion) have identified 2943, 2242, 3162, 2187 and 2028 proteins respectively, yielding 5040 proteins in total. Therefore, PEG precipitation followed by albumin depletion should be used as a general strategy to expand the MS-based plasma proteome coverage for blood biomarker discoveries; and our datasets in this study provide as resources for increased chances of detecting proteins of interests in the whole plasma or particular fractions. |
HostingRepository | iProX |
AnnounceDate | 2025-01-26 |
AnnouncementXML | Submission_2025-01-27_03:07:42.710.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bing Bai |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-01-27 03:07:18 | ID requested | |
⏵ 1 | 2025-01-27 03:07:43 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Polyethylene glycol, albumin depletion, plasma, proteomics |
Contact List
Bing Bai | |
---|---|
contact affiliation | Nanjing University |
contact email | bing.bai@nju.edu.cn |
lab head | |
Bing Bai | |
contact affiliation | Nanjing University |
contact email | bing.bai@nju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |