PXD060235

PXD060235 is an original dataset announced via ProteomeXchange.

Title	PEG6000 precipitation and albumin depletion for extended plasma proteomics analysis
Description	The Mass spectrometry (MS)-based blood plasma or serum proteome analysis is limited by interference from albumin, immunoglobins and other commonly abundant proteins. We have found that polyethylene glycol (PEG) can efficiently precipitate immunoglobins while leave albumin largely in the supernatant. By PEG precipitation followed by albumin depletion, an increased number of proteins and N-glycoproteins can be identified by LC-MS/MS from the plasma. In-depth LC-LC-MS/MS proteomic analyses of the plasma and its four fractionated samples (10% and further 20% PEG-precipitated pellets and their supernatants with albumin depletion) have identified 2943, 2242, 3162, 2187 and 2028 proteins respectively, yielding 5040 proteins in total. Therefore, PEG precipitation followed by albumin depletion should be used as a general strategy to expand the MS-based plasma proteome coverage for blood biomarker discoveries; and our datasets in this study provide as resources for increased chances of detecting proteins of interests in the whole plasma or particular fractions.
HostingRepository	iProX
AnnounceDate	2025-01-26
AnnouncementXML	Submission_2025-01-27_03:07:42.710.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bing Bai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 03:07:18	ID requested
⏵ 1	2025-01-27 03:07:43	announced

Dataset with its publication pending

submitter keyword: Polyethylene glycol, albumin depletion, plasma, proteomics

Bing Bai
contact affiliation	Nanjing University
contact email	bing.bai@nju.edu.cn
lab head
Bing Bai
contact affiliation	Nanjing University
contact email	bing.bai@nju.edu.cn
dataset submitter

iProX dataset URI