PXD060229

PXD060229 is an original dataset announced via ProteomeXchange.

Title	Gut microbiota induce neutrophil activation that aggravates stroke pathology
Description	Neutrophils, key players in the innate immune system, become activated following ischemic stroke. However, the influence of gut microbiota on neutrophil activation and its impact on inflammatory brain injury is not yet fully understood. In this study, we demonstrate that microbiota colonization in germ-free mice activates neutrophils and worsens disease outcomes. To investigate the phenotypic and molecular alterations in neutrophils, as well as their activity in stroke mice with either depleted or intact microbiota, we conducted a comparative proteomic analysis using liquid chromatography-based mass spectrometry. Our findings revealed that microbiota depletion leads to a downregulation of inflammatory proteins in circulating neutrophils and a reduction in neutrophil extracellular traps (NETs). A similar pattern was observed in neutrophils infiltrating the brain, accompanied by a decrease in infarct size and alleviated behavioral deficits.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-01_16:19:10.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susmita Ghosh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 02:55:08	ID requested
⏵ 1	2025-06-01 16:19:11	announced

Tuz AA, Ghosh S, Karsch L, Antler M, Lakovic V, Lohmann S, Lehmann AH, Beer A, Nagel D, Jung M, H, ö, renbaum N, Kaygusuz V, Qefalia A, Alshaar B, Amookazemi N, Bolsega S, Basic M, Siveke JT, Heiles S, Gr, ü, neboom A, Lueong S, Herz J, Sickmann A, Hagemann N, Hasenberg A, Hermann DM, Gunzer M, Singh V, Gut microbiota deficiency reduces neutrophil activation and is protective after ischemic stroke. J Neuroinflammation, 22(1):137(2025) [pubmed]

10.1186/s12974-025-03448-w;

submitter keyword: Gut microbiota, proteomics, stroke,Neutrophil

Albert Sickmann
contact affiliation	Bioanalytik, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany
contact email	sickmann@isas.de
lab head
Susmita Ghosh
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	susmita.ghosh@isas.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060229
     1. Label: PRIDE project
     2. Name: Gut microbiota induce neutrophil activation that aggravates stroke pathology