PXD060228

PXD060228 is an original dataset announced via ProteomeXchange.

Title	Insulin-degrading enzyme regulates mRNA processing and in-teracts with the CCR4-NOT transcription complex
Description	Insulin-degrading enzyme is zinc metallo protease degrading low molecular weight substrates including insulin. Ubiquitous expression, high evolutionary conservation, upregulation of Ide in stress situations, and various literature findings suggest a broader function of Ide in cell physiology and protein homeostasis that remains to be elucidated. We used proteomics and transcriptomics approaches searching for leads related to a broader role of Ide in protein homeostasis. We combined an analysis of the proteome and single-cell transcriptome of Ide+/+ and Ide-/- pancreatic islet cells with an examination of the interactome of human cytosolic Ide using proximity biotinylation. We observe an upregulation of pathways related to RNA processing and translation in Ide+/+ relative to Ide-/- islet cells. Corroborating these results and providing a potential mechanistic explanation, proximity biotinylation reveals interaction of Ide with several subunits of CCR4-NOT, a key complex and mRNA deadenylase regulating protein expression "from birth to death". We propose a speculative model in which Ide and CCR4-NOT cooperate to control and limit protein expression in proteotoxic and met-abolic stress situations through cooperation between their deadenylase and protease functions.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:14:56.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-27 02:42:03	ID requested
⏵ 1	2025-06-15 16:14:56	announced

10.3390/cells14110792;

Bertocci B, Yilmaz A, Waeckel-, É, n, é, e E, Guerrera C, Roger K, Tour, é L, van Endert PM, Insulin-Degrading Enzyme Regulates mRNA Processing and May Interact with the CCR4-NOT Complex. Cells, 14(11):(2025) [pubmed]

submitter keyword: Insulinase

protein homeostasis

RNA processing

CCR4-NOT

beta cell

islet of Langerhans, timsTOF Pro

Ida Chiara Guerrera
contact affiliation	Proteomics Platform Necker, Université Paris Cité - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UAR3633, Paris 75015, France.
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD060228

PRIDE project URI

• PRIDE
  1. PXD060228
     1. Label: PRIDE project
     2. Name: Insulin-degrading enzyme regulates mRNA processing and in-teracts with the CCR4-NOT transcription complex