PXD060219

PXD060219 is an original dataset announced via ProteomeXchange.

Title	Development and proteome characterisation of muscle cell culture derived from Genetically improved farmed tilapia, Oreochromis niloticus (Linnaeus,1758)
Description	A quantitative proteomics approach was used to investigate in Oreochromis niloticus cell line across two passages P10, and P20. A cell line developed from the muscle tissue of O. niloticus (ONM) was utilized to understand the proteome in-depth. Comparative proteome analysis was performed for ONM muscle cells between two passages (10 and 20) using label free quantitative proteomics. High-resolution mass spectrometry was used for data using label-free quantification. Data were further analysed using a statistical tool across passages of rohu muscle cell line. Proteomic analysis across two passages (P10 and P20) revealed pathways involved in myogenesis were striated muscle contraction, muscle contraction, insulin pathway, regulation of actin cytoskeleton, hedgehog on state, VEGFA-VEGFR2 and signalling by WNT. The cell line generated can have wide range of application in cultivated meat as well as in in vitro application.
HostingRepository	PRIDE
AnnounceDate	2026-04-23
AnnouncementXML	Submission_2026-04-23_09:00:40.025.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD060219
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nevil Pinto
SpeciesList	scientific name: Oreochromis niloticus; NCBI TaxID: NEWT:8128;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-26 21:56:00	ID requested
⏵ 1	2026-04-23 09:00:40	announced

10.1016/J.DIFF.2026.100934;

10.6019/PXD060219;

submitter keyword: Oreochromis niloticus, Muscle cell culture, myogenesis, LC-MS/MS

Mukunda Goswami
contact affiliation	ICAR-CIFE Mumbai 400061
contact email	mukugoswami@gmail.com
lab head
Nevil Pinto
contact affiliation	Mangalore University, Mangalore
contact email	nevilpinto1@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060219
     1. Label: PRIDE project
     2. Name: Development and proteome characterisation of muscle cell culture derived from Genetically improved farmed tilapia, Oreochromis niloticus (Linnaeus,1758)