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PXD060175
PXD060175 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A multi-omic approach reveals iron availability influences hepatocyte cell fate |
| Description | Recent evidence has highlighted the importance of employing cell culture media designed to reproduce the physiological metabolic environment in vitro. Here, we utilize the physiological medium Plasmax to examine the impact of nutrient availability on the model human hepatocyte cell line, HepG2. Incubation of HepG2 cells in Plasmax suppressed a transcriptional program driven by Hepatocyte Nuclear Factor 4 (HNF4A), a master regulator of hepatocyte identity. Given that HepG2 cells were originally isolated from a patient with hepatoblastoma, this suggests reversion to the native state in physiological media. Importantly, exclusion of the trace element iron from Plasmax reinstated the HNF4A-driven transcriptional program. Taken together, these studies suggest a relationship between iroN availability and regulation of hepatocyte cell fate and highlight the importance of more faithfully recapitulating in vivo metabolite availability in vitro. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-16 |
| AnnouncementXML | Submission_2026-03-16_06:19:58.380.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD060175 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Kristin Brown |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-24 16:14:12 | ID requested | |
| ⏵ 1 | 2026-03-16 06:19:59 | announced |
Publication List
| 10.6019/PXD060175; |
| Ong AJS, Tigani TA, Gomes AJ, Reinecke JM, Cox AG, Brown KK, A multi-omic approach reveals iron availability influences cell fate fidelity. NPJ Metab Health Dis, 4(1):(2026) [pubmed] |
| 10.1038/s44324-026-00102-8; |
Keyword List
| submitter keyword: Liver, Plasmax, HepG2 |
Contact List
| Kristin K Brown | |
|---|---|
| contact affiliation | Peter MacCallum Cancer Centre, Australia |
| contact email | kristin.brown@petermac.org |
| lab head | |
| Kristin Brown | |
| contact affiliation | Peter MacCallum Cancer Centre and The University of Melbourne |
| contact email | kristin.brown@petermac.org |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD060175 |
| PRIDE project URI |
Repository Record List
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