PXD060168 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isotopic labeling-enabled Chemical Proteomic Analysis Revealed Structural and Functional Features of Allysine Modifications in Mammalian Cells and Tissues |
Description | In this study, we reported the application of a streamlined chemical proteomic workflow with heavy isotopic labeling that enables system-wide enrichment and site-specific identification of allysine as well as other carbonylated peptides such as peptides containing glutamic semialdehyde derived from oxidative damage of arginine and proline with high confidence in cultured human cells and mouse tissues. The streamlined workflow led to the identification of 434 allysine sites on 349 proteins in human 293T and HCT116 cells and 317 allysine sites on 157 proteins in mouse muscle tissues without any treatment of oxidative stress-inducing chemical reagent. We identified 48 histone allysine sites including 38 sites on core histones in human 293T cells, many of which overlapped with well-characterized histone acetylation and methylation epigenetic marks. Bioinformatic analysis revealed notable characteristics of the amino acid preferences of allysine flanking sequences and significant depletion of allysine sites in the protein secondary structures in human cells. Pathway analysis showed that allysine substrates were involved in diverse cellular processes including translation, protein folding and RNA processing in human cells and were enriched with muscle contractile fiber proteins and metabolic enzymes in mouse muscle tissue. Thus, our integrated chemical proteomic analysis revealed structural and functional features of allysine targets under regular growth conditions in mammalian cells and tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-29 |
AnnouncementXML | Submission_2025-04-29_01:59:06.605.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yue Chen |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | L-allysine; acetylated residue; glutamyl semialdehyde |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-01-24 11:17:41 | ID requested | |
1 | 2025-04-22 14:33:21 | announced | |
⏵ 2 | 2025-04-29 01:59:07 | announced | 2025-04-29: Updated project metadata. |
Publication List
Keyword List
submitter keyword: carbonylation, lysyl oxidase, chemical proteomics, isotopic labeling,allysine |
Contact List
Yue Chen |
contact affiliation | University of Minnesota |
contact email | yuechen@umn.edu |
lab head | |
Yue Chen |
contact affiliation | University of Minnesota |
contact email | ychen2001@yahoo.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD060168
- Label: PRIDE project
- Name: Isotopic labeling-enabled Chemical Proteomic Analysis Revealed Structural and Functional Features of Allysine Modifications in Mammalian Cells and Tissues