PXD060124

PXD060124 is an original dataset announced via ProteomeXchange.

Title	Alterations in Proteomic and Metabolomic Profiles of Cryptococcus neoformans following Hsl101 and Urk1 Deletion
Description	Cryptococcus neoformans is a significant human fungal pathogen, and its kinases, Hsl101 and Urk1, play pivotal roles in virulence during lung and brain infections. This study systematically investigates the effects of Hsl101 and Urk1 deletions on the proteomic and metabolomic profiles of C. neoformans. By generating deletion mutants for Hsl101, we identified profound alterations in the expression levels of 366 proteins and 421 metabolites, including those involved in oxidative phosphorylation and ATP synthesis. Similarly, deletion of Urk1 resulted in significant changes in the expression of 236 proteins and 366 metabolites, highlighting pathways such as steroid biosynthesis and starch and sucrose metabolism. These findings not only elucidate the regulatory roles of Hsl101 and Urk1 in the metabolic pathways of C. neoformans but also pave the way for potential therapeutic interventions targeting these kinases.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_07:03:35.021.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng Xue
SpeciesList	scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-23 09:56:00	ID requested
⏵ 1	2025-05-07 07:03:35	announced

Ma Y, Xue P, . Int J Mol Sci, 26(5):(2025) [pubmed]

10.3390/ijms26051824;

submitter keyword: Cryptococcus neoformans

Kinase

Hsl101

Urk1

Proteome

Metabolomics

Peng Xue
contact affiliation	Nantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, China
contact email	pengxue@ntu.edu.cn
lab head
Peng Xue
contact affiliation	https://www.ebi.ac.uk/pride/
contact email	pengxue@ntu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD060124

PRIDE project URI

• PRIDE
  1. PXD060124
     1. Label: PRIDE project
     2. Name: Alterations in Proteomic and Metabolomic Profiles of Cryptococcus neoformans following Hsl101 and Urk1 Deletion