PXD060099

PXD060099 is an original dataset announced via ProteomeXchange.

Title	The HAUS6 CH domain binds microtubules to orient augmin for microtubule branching
Description	Branching microtubule nucleation is a key mechanism for mitotic and meiotic spindle assembly and requires the hetero-octameric augmin complex. Augmin recruits the major microtubule nucleator, the γ-tubulin ring complex, to pre-existing microtubules to direct the formation of new microtubules in a defined orientation. Although recent structural work has provided key insights into augmin’s structural organization, molecular details of its interaction with microtubules remain elusive. Here, we identify the minimal conserved microtubule-binding unit of augmin across species and demonstrate that the low-complexity microtubule-binding unit from D. melanogaster mediates microtubule binding predominantly via the calponin homology (CH) domain in Dgt6/HAUS6. Comparative sequence and functional analyses reveal a highly conserved architecture of the HAUS6 CH domain and variations in the importance of the HAUS8 N-terminus in microtubule binding. Using cryo-electron microscopy and molecular dynamics simulations, we show that the HAUS6 CH domain binds at the inter-protofilament groove between two adjacent β-tubulin subunits and thereby orients augmin on microtubules. Altogether, our findings reveal how augmin binds microtubules to pre-determine the branching angle during microtubule nucleation and thereby facilitates the rapid assembly of complex microtubule networks.
HostingRepository	PRIDE
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_00:07:47.805.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcin Luzarowski
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-23 00:53:06	ID requested
⏵ 1	2025-07-30 00:07:48	announced

Dataset with its publication pending

submitter keyword: microtubule nucleation,Microtubules, cell division

Marcin Luzarowski
contact affiliation	Core Facility for Mass Spectrometry & Proteomics Center for Molecular Biology of Heidelberg University (ZMBH 69120 Heidelberg
contact email	m.luzarowski@zmbh.uni-heidelberg.de
lab head
Marcin Luzarowski
contact affiliation	University of Heidelberg
contact email	m.luzarowski@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD060099

PRIDE project URI

• PRIDE
  1. PXD060099
     1. Label: PRIDE project
     2. Name: The HAUS6 CH domain binds microtubules to orient augmin for microtubule branching