PXD060092

PXD060092 is an original dataset announced via ProteomeXchange.

Title	Evaluation of serum supplementation on the development of Haemonchus contortus larvae in vitro and on compound screening results
Description	A high-throughput platform for assessing the activity of synthetic or natural com-pounds on the motility and development of Haemonchus contortus larvae has been estab-lished for identifying new anthelmintic compounds active against strongylid nema-todes. This study evaluated the impact of serum supplementation on larval develop-ment, motility and survival in vitro and its implications for phenotypic compound screening. Of five blood components assessed, 7.5% sheep serum significantly enhanced larval development, motility and survival compared with the original medium (LB*), leading to the formulation of an improved medium (LBS*). Proteomic analysis revealed marked differences in protein expression in larvae cultured in LBS* versus LB*, includ-ing molecules associated with structural integrity and metabolic processes. The pheno-typic screening of 240 compounds (“Global Priority Box” from Medicines Malaria Ven-ture) using LBS* yielded results distinct from those in LB*, highlighting the effect of culture conditions on screening assessments. These findings indicate/emphasise the critical need to evaluate and optimise culture media for physiologically conditions in screening platforms, improving the reliability of anthelmintic discovery.
HostingRepository	PRIDE
AnnounceDate	2025-02-27
AnnouncementXML	Submission_2025-02-26_19:14:25.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Wang
SpeciesList	scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-22 19:06:03	ID requested
⏵ 1	2025-02-26 19:14:26	announced

10.3390/ijms26031118;

Thilakarathne SS, Taki AC, Wang T, Nowell C, Chang BCH, Gasser RB, Larvae In Vitro and on Compound Screening Results. Int J Mol Sci, 26(3):(2025) [pubmed]

submitter keyword: Haemonchus contortus

parasitic nematode

in vitro culture

serum supplementation

an-thelmintic screening

Robin B. Gasser
contact affiliation	Melbourne Veterinary School, Faculty of Science, The University of Melbourne
contact email	robinbg@unimelb.edu.au
lab head
Tao Wang
contact affiliation	The University of Melbourne
contact email	tao.wang1@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD060092

PRIDE project URI

• PRIDE
  1. PXD060092
     1. Label: PRIDE project
     2. Name: Evaluation of serum supplementation on the development of Haemonchus contortus larvae in vitro and on compound screening results