PXD060078

PXD060078 is an original dataset announced via ProteomeXchange.

Title	Chemical crosslinking of plastocyanin and the cytochrome b6f complex
Description	In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well characterized, the interaction between PC and Cytf remains less clear. Using chemical crosslinking combined with mass spectrometry, we identified two potential binding models for PC and Cytf: Side-on and Head-on.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_18:17:58.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Scholz
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-22 12:59:57	ID requested
⏵ 1	2025-08-24 18:17:59	announced

10.1093/plphys/kiaf269;

Milrad Y, Wegemann D, Kuhlgert S, Scholz M, Younas M, Vidal-Meireles A, Hippler M, Insights into plastocyanin-cytochrome b6f complex formation: The role of plastocyanin phosphorylation. Plant Physiol, 198(4):(2025) [pubmed]

submitter keyword: Cytochrome b6f,Plastocyanin, Chlamzdomonas, EDC, crosslinking

Michael Hippler
contact affiliation	University of Muenster, Institute of Plant Biology and Biotechnology
contact email	mhippler@uni-muenster.de
lab head
Martin Scholz
contact affiliation	IBBP Muenster
contact email	mscholz@uni-muenster.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD060078

PRIDE project URI

• PRIDE
  1. PXD060078
     1. Label: PRIDE project
     2. Name: Chemical crosslinking of plastocyanin and the cytochrome b6f complex