PXD060077

PXD060077 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Receptive and Non-receptive Endometria-derived Uterine Fluid by LC-MS/MS
Description	The current diagnostic tools used to determine the precise timing of embryo transfer for in vitro fertilization (IVF)-frozen embryo transfer (FET) patients are not non-invasive or accurate. As uterine fluid is collected via a minimally invasive procedure and reflects alterations in the molecular composition of the endometrium during the menstrual cycle, it can be considered an accurate diagnostic source for identifying endometrial receptivity-related proteins. Therefore, we aimed to identify a panel of molecular biomarkers for endometrial receptivity by investigating uterine fluid lavage (UFL). The UFLs of 18 IVF-FET patients were collected two days before FET. After seven weeks, the patients were divided into receptive and non-receptive groups on the basis of ultrasonography results for clinical pregnancy. Protein profiling of the collected UFLs was performed via tandem mass tag labeling and mass spectrometry liquid chromatography. Next, differentially abundant proteins between the two groups were identified. Additionally, the largest contributions of the variable to the differences based on the variable of importance in projection (VIP) score value were inspected. The candidate protein panel was selected on the basis of the VIP score and protein database analysis. The accuracy of the candidate panel was assessed via logistic regression as a machine learning approach. Finally, western blot analysis confirmed the results.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-02_04:22:34.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnoosh Saraee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-22 12:56:11	ID requested
⏵ 1	2025-06-02 04:22:35	announced

Dataset with its publication pending

submitter keyword: Human, Endometrial Receptivity Assessment, Biomarker., Embryo Implantation, Uterine Fluid Lavage, Proteomics

Ghasem Hosseini Salekdeh
contact affiliation	Department of Molecular Sciences, Macquarie University, North Ryde, NSW, Australia
contact email	Hosseini.salekdeh@mq.edu.au
lab head
Farnoosh Saraee
contact affiliation	Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
contact email	farnoosh.saraee@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD060077

PRIDE project URI

• PRIDE
  1. PXD060077
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Receptive and Non-receptive Endometria-derived Uterine Fluid by LC-MS/MS