⮝ Full datasets listing
PXD060029
PXD060029 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Snx17 facilitates bunyavirus infection |
| Description | Rift Valley Fever Virus (RVFV) is a negative-strand RNA bunyavirus with a tri-segmented genome and that can infect humans and animals due to its broad tropism. Through a loss-of-function genome-wide CRISPR Cas9 screen, we previously identified Lrp1 in RVFV entry facilitated by the viral glycoproteins. This screen also identified several additional host factors that are important for viral infection. Here, we describe our efforts to define a role for Sorting Nexin 17 (Snx17) in bunyavirus infection. Our findings reveal that the removal of Snx17 results in a substantial reduction of RVFV infection in Snx17 knock-out cells. Further analysis revealed that Snx17 removal leads to a reduction in the Lrp1 levels, but not complete loss of Lrp1 expression. We also show that Snx17 is essential for the entry and infection of several bunyaviruses that do not utilize Lrp1, suggesting a broader impact on viral entry. These results support a role where Snx17 can facilitate viral infection in an Lrp1-dependent and an Lrp1-independent manner. Our findings highlight how bunyaviruses use multiple host factors to gain cellular entry and identify additional pathways that can be used to dissect entry mechanisms and targeted for therapeutic intervention. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-09-02 |
| AnnouncementXML | Submission_2026-09-02_12:17:28.580.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Cho Byoung-Kyu |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | TMT6plex |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-21 11:40:43 | ID requested | |
| ⏵ 1 | 2026-09-02 12:17:29 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TMT, DatasetType:Proteomics |
Contact List
| Young Ah Goo | |
|---|---|
| contact affiliation | Washington University in St. Louis |
| contact email | ygoo@wustl.edu |
| lab head | |
| Cho Byoung-Kyu | |
| contact affiliation | Washington University in St. Louis |
| contact email | byoung-kyu@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000096904/ |
