PXD060014

PXD060014 is an original dataset announced via ProteomeXchange.

Title	CPK3 phosphorylates SNC1-TIR LC-MSMS
Description	Toll/interleukin-1 receptor (TIR) domain proteins are immune signaling components and function as NAD+-cleaving enzymes to activate defense responses. Activation of TIRs represses growth and drives cell death in plants and promotes axon degeneration in animals, but how plant TIRs are repressed remains unclear. Here, we show that TIR NADase activity requires a conserved serine residue spatially close to the catalytic glutamate. The plant Ca2+-dependent protein kinases (CPKs), the mammalian Ca2+/calmodulin-dependent protein kinase II delta (CAMK2D) and TANK binding kinase 1 (TBK1) phosphorylate TIR domains at this conserved serine, which blocks TIR NADase activities and functions and thus maintains growth in plants and suppresses SARM1 TIR signaling in animals, respectively. Our findings define a fundamental molecular mechanism by which phosphorylation at a conserved serine residue blocks TIR signaling to balance growth and defense trade-offs.
HostingRepository	PRIDE
AnnounceDate	2025-06-12
AnnouncementXML	Submission_2025-06-12_03:55:59.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Yu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-21 04:28:17	ID requested
⏵ 1	2025-06-12 03:55:59	announced

10.1038/s41477-025-02012-x;

Li J, Chen S, Yu B, Li Q, Liu R, Wang Z, Wan L, Zhao Y, TIR immune signalling is blocked by phosphorylation to maintain plant growth. Nat Plants, 11(6):1193-1204(2025) [pubmed]

submitter keyword: LC-MSMS,CPK3, SNC1-TIR

Yang Zhao
contact affiliation	CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
contact email	zhaoyang@cemps.ac.cn
lab head
Bo Yu
contact affiliation	CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
contact email	boyu@cemps.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD060014

PRIDE project URI

• PRIDE
  1. PXD060014
     1. Label: PRIDE project
     2. Name: CPK3 phosphorylates SNC1-TIR LC-MSMS