PXD060010

PXD060010 is an original dataset announced via ProteomeXchange.

Title	Identification of OCEL1-binding proteins from Escherichia coli by mass spectrometry
Description	To identify OCEL1-binding proteins in Escherichia coli, we set two experimental groups (A and B). Groups A and B represented the expression of GST protein alone or GST-OCEL1-P81A fusion protein in bacteria respectively. The cultures of E. coli BL21 transformed with pGEX-4T1 or pGEX-4T1-OCEL1-P81A were induced with 0.3 mM isopropyl-β-D-thiolgalactopyranoside (IPTG) at 18°C for 6 h. The harvested bacteria were suspended in PBS containing 1% Triton X-100 (PBST) and protease inhibitors and then lysed by sonication. GST resins were added to the bacterial lysates and mixed overnight at 4°C. After 4 washes with PBS, the enriched proteins were were subjected to 10% SDS-PAGE gel. After Coomassie Blue staining, bands corresponding to each group were collected and analyzed by mass spectrometry(LC-MS/MS) in ProteinGene Biotech (Wuhan, Hubei, China). All acquired raw data were processed with pFind software (version 3.1.2) and searched against the NCBI E. coli protein database (https://www.ncbi.nlm.nih. gov/protein). After identifying the associated proteins in each group, we compared them with each other. The proteins enriched in group B compared with those in group A were considered as OCEL1 associated proteins.
HostingRepository	iProX
AnnounceDate	2025-01-21
AnnouncementXML	Submission_2025-01-21_00:06:30.952.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhi Li
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-21 00:05:49	ID requested
⏵ 1	2025-01-21 00:06:31	announced

Dataset with its publication pending

submitter keyword: OCEL1-binding proteins

Wuhan Xiao
contact affiliation	Institute of Hydrobiology, Chinese Academy of Sciences
contact email	w-xiao@ihb.ac.cn
lab head
Zhi Li
contact affiliation	Institute of Hydrobiology, Chinese Academy of Sciences
contact email	lizhi73@ihb.ac.cn
dataset submitter

iProX dataset URI