PXD060004

PXD060004 is an original dataset announced via ProteomeXchange.

Title	CRISPR-edited iPSCs reveal BSN gene mutations induce neuronal hyperexcitability via astrocyte lipid accumulation
Description	BSNM1903V / wt, BSNM1903V / M1903V, BSN 5672insCG / wt were found in several patients with epilepsy. CRISPR / Cas9 technology was used to generate BSN mutant iPSCs to explore the mechanism of epilepsy. BSN mutant iPSCs differentiate into neural stem cells. Proteomic sequencing showed that there were significant differences in lipid metabolism pathways. By further differentiation into astrocytes, it was further verified that BSN mutation stimulated excessive excitement of astrocytes through lipid accumulation, resulting in neuronal damage and seizures.
HostingRepository	iProX
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-18_06:43:22.727.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinliang Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-20 19:32:11	ID requested
⏵ 1	2025-09-18 06:43:23	announced

Dataset with its publication pending

submitter keyword: BSN, lipid accumulation, epilepsy, neural stem cells

Jinliang Li
contact affiliation	Zhanjiang Central Hospital, Guangdong Medical University
contact email	shzlijinliang@bjmu.edu.cn
lab head
Jinliang Li
contact affiliation	Zhanjiang Central Hospital, Guangdong Medical University
contact email	shzlijinliang@bjmu.edu.cn
dataset submitter

iProX dataset URI