PXD059985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mitochondrial metabolism and hypoxic signaling in differentiated human cardiomyocyte AC16 cell line |
Description | Cardiovascular diseases are associated with an altered cardiomyocyte metabolism. Due to a shortage of human heart tissue, experimental studies mostly rely on alternative approaches including animal and cell culture models. Since the use of isolated primary cardiomyocytes is limited, immortalized cardiomyocyte cell lines may represent a useful tool as they closely mimic human cardiomyocytes. This study is focused on the AC16 cell line generated from adult human ventricular cardiomyocytes. Despite an increasing number of articles employing AC16 cells, the comprehensive proteomic, bioenergetic and oxygen-sensing characterization of proliferating versus differentiated cells is still lacking. Here, we provide a comparison of these two stages, particularly emphasizing cell metabolism, mitochondrial function, and hypoxic signalling. The label-free quantitative mass spectrometry revealed a decrease in autophagy and cytoplasmic translation in differentiated AC16, confirming their phenotype. Cell differentiation led to the global increase in mitochondrial proteins (e.g. OXPHOS proteins, TFAM, VWA8, etc.) reflected by elevated mitochondrial respiration. Fatty acid oxidation proteins were increased in differentiated cells, while the expression levels of proteins associated with fatty acid synthesis were unchanged, and glycolytic proteins were decreased. There was a profound difference between proliferating and differentiated cells in their response to hypoxia and anoxia/reoxygenation. We conclude that AC16 differentiation leads to proteomic and metabolic shifts and altered cell response to oxygen deprivation. This underscores the requirement for proper selection of particular differentiation state during experimental planning. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-14 |
AnnouncementXML | Submission_2025-04-14_03:48:08.436.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marek Vrbacky |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-01-20 09:58:15 | ID requested | |
⏵ 1 | 2025-04-14 03:48:08 | announced | |
Publication List
Keyword List
submitter keyword: metabolism, hypoxia, differentiation, mitochondria,AC16 cardiomyocytes |
Contact List
Lukas Alan |
contact affiliation | Institute of Physiology, Czech Academy of Sciences Prague, Czech Republic |
contact email | Lukas.Alan@fgu.cas.cz |
lab head | |
Marek Vrbacky |
contact affiliation | Czech Academy of Sciences |
contact email | proteom.krc@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059985
- Label: PRIDE project
- Name: Mitochondrial metabolism and hypoxic signaling in differentiated human cardiomyocyte AC16 cell line