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PXD059985

PXD059985 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial metabolism and hypoxic signaling in differentiated human cardiomyocyte AC16 cell line
DescriptionCardiovascular diseases are associated with an altered cardiomyocyte metabolism. Due to a shortage of human heart tissue, experimental studies mostly rely on alternative approaches including animal and cell culture models. Since the use of isolated primary cardiomyocytes is limited, immortalized cardiomyocyte cell lines may represent a useful tool as they closely mimic human cardiomyocytes. This study is focused on the AC16 cell line generated from adult human ventricular cardiomyocytes. Despite an increasing number of articles employing AC16 cells, the comprehensive proteomic, bioenergetic and oxygen-sensing characterization of proliferating versus differentiated cells is still lacking. Here, we provide a comparison of these two stages, particularly emphasizing cell metabolism, mitochondrial function, and hypoxic signalling. The label-free quantitative mass spectrometry revealed a decrease in autophagy and cytoplasmic translation in differentiated AC16, confirming their phenotype. Cell differentiation led to the global increase in mitochondrial proteins (e.g. OXPHOS proteins, TFAM, VWA8, etc.) reflected by elevated mitochondrial respiration. Fatty acid oxidation proteins were increased in differentiated cells, while the expression levels of proteins associated with fatty acid synthesis were unchanged, and glycolytic proteins were decreased. There was a profound difference between proliferating and differentiated cells in their response to hypoxia and anoxia/reoxygenation. We conclude that AC16 differentiation leads to proteomic and metabolic shifts and altered cell response to oxygen deprivation. This underscores the requirement for proper selection of particular differentiation state during experimental planning.
HostingRepositoryPRIDE
AnnounceDate2025-04-14
AnnouncementXMLSubmission_2025-04-14_03:48:08.436.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarek Vrbacky
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-01-20 09:58:15ID requested
12025-04-14 03:48:08announced
Publication List
10.1152/AJPCELL.00083.2025;
Keyword List
submitter keyword: metabolism, hypoxia, differentiation, mitochondria,AC16 cardiomyocytes
Contact List
Lukas Alan
contact affiliationInstitute of Physiology, Czech Academy of Sciences Prague, Czech Republic
contact emailLukas.Alan@fgu.cas.cz
lab head
Marek Vrbacky
contact affiliationCzech Academy of Sciences
contact emailproteom.krc@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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