PXD059979 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemical probe-enabled lipid droplet proteomics |
Description | Emerging evidence indicates that lipid droplets (LDs) play important roles in lipid metabolism, energy homeostasis, and cell stress management. Notably, dysregulation of LDs is tightly linked to numerous diseases, including lipodystrophies, cancer, obesity, atherosclerosis and others. The pivotal physiological roles of LDs have led to an exploration of research in recent years. The functions of LDs are inherently connected to the composition of their proteome. Current methods for profiling LD proteins mostly utilize LD fractionation including those based on proximity-based labeling techniques. Glob-al profiling of the LD proteome in live cells without isolation of LDs is still challenging. Herein, we disclose two small-molecule chemical probes, termed LDF and LDPL. Both LDF/LDPL are small in size, could freely and specifically migrate within the lipid context of LDs. Consequently, they were successfully used for live-cell fluorescence imaging of LDs and from animal tissues. We further showed that LDPL was capable of large-scale profiling of LD proteome without the need of LD isolation. By using LDPL, 1549 high-confidence proteins, most of which could be annotated to prominent LD func-tions, were next identified. Importantly, further validation studies by using representative “hit” proteins revealed that CHMP6 and PRDX4 could act as the lipophagy receptor and lipolysis suppressor, respectively. Our results thus confirmed for the first time that LDPL is a powerful chemical tool for in situ profiling of LD proteomes. With the ability to provide a deeper understanding of LD proteomics from the native cellular environments, our newly developed strategy may be used in future to decipher the dynamics and molecular mechanism of LDs in various diseases. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-27 |
AnnouncementXML | Submission_2025-05-27_08:06:34.367.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Junzhe Zhang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-01-20 07:58:32 | ID requested | |
⏵ 1 | 2025-05-27 08:06:34 | announced | |
Publication List
Keyword List
submitter keyword: Fluorescence imaging,Lipid droplet, Proteomic, Chemical probe, Labeling |
Contact List
Jigang Wang |
contact affiliation | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences |
contact email | jgwang@icmm.ac.cn |
lab head | |
Junzhe Zhang |
contact affiliation | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences |
contact email | jzzhang@icmm.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059979
- Label: PRIDE project
- Name: Chemical probe-enabled lipid droplet proteomics