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PXD059967
PXD059967 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Electric pulse stimulation in human muscle cells |
| Description | Electrical pulse stimulation (EPS) is used to study exercise-related adaptations in muscle cells in vitro. We compared the effects of two EPS protocols: 1. intermittent high-frequency or 2. continuous low-frequency stimulation, on glucose and fatty acid metabolism, muscle fiber-type markers, mitochondrial proteins, myokines and extracellular vesicles (EV) release. Both EPS protocols enhanced post-exercise glycogen synthesis, while an increase in oxidative capacity was observed only after the intermittent high-frequency stimulation. Both EPS protocols increased GDF11 protein and release of EVs, which were characterized by transmission electron microscopy, nanoparticle tracking analysis and proteomics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-12 |
| AnnouncementXML | Submission_2026-01-11_17:53:25.717.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Václav Pustka |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-20 03:50:42 | ID requested | |
| ⏵ 1 | 2026-01-11 17:53:26 | announced |
Publication List
| 10.1002/2211-5463.70114; |
| Gabri, š, ov, á K, Kurdiov, á T, Barkova D, P, á, le, š, ov, á N, Babulicov, á J, Ty, č, iakov, á S, Novotov, á M, Bal, á, ž, ov, á M, Sabo M, Pustka V, Ukropec J, Ukropcov, á B, Electrical pulse stimulation reflecting the episodic nature of real-life exercise modulates metabolic and secretory profile of primary human myotubes. FEBS Open Bio, 16(1):127-144(2026) [pubmed] |
Keyword List
| submitter keyword: metabolism, myokines, extracellular vesicles, EPS, human muscle cells,Electric pulse stimulation |
Contact List
| Zbynek Zdrahal | |
|---|---|
| contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
| contact email | zdrahal@sci.muni.cz |
| lab head | |
| Václav Pustka | |
| contact affiliation | Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic |
| contact email | vaclav.pustka@ceitec.muni.cz |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD059967 |
| PRIDE project URI |
Repository Record List
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