PXD059934

PXD059934 is an original dataset announced via ProteomeXchange.

Title	Small Extracellular Vesicles Derived from Human Trabecular Meshwork Stem Cells are distinct from TM Cells in their Proteome Cargo and Function
Description	Glaucoma is an optic neuropathy, the second leading cause of irreversible blindness worldwide. In our earlier studies, we demonstrated the isolation and functional efficacy (wound healing and anti-oxidant potential) of small extracellular vesicles (sEV) from the adult tissue-resident stem cells of the trabecular meshwork (TMSC). This study aimed to elucidate the protein profile of sEV derived from TMSCs to correlate these functional differences for a better understanding of their potential as a cell-free therapeutic agents for primary open angle glaucoma (POAG). TM and TMSC sEV were isolated by ultracentrifugation and characterized. The protein cargo of these sEV were analyzed by Mass spectrometry. Mass spectrometry analysis identified 2802 proteins in TMSC sEV and 2848 in TM sEV. Further Maxquant analysis revealed that 511 proteins were reproducibly quantified across the samples. and 18% of the proteins were significantly altered between TMSC and TM sEV. Differential expression analysis identified distinct protein profiles between TMSC and TM sEV. Notably, sEV from TMSCs were enriched with proteins associated with wound healing, cell proliferation, migration, anti-oxidant and anti-apoptotic activities, consistent with the findings in other mesenchymal stem cells. Pathway analysis highlighted the enrichment of proteins associated with PI3K-AKT and MAPK signaling pathways. Further validation by western blotting confirmed that TMSC sEV effectively modulated these pathways in TM cells, which are essential for cell proliferation and survival under oxidative stress. To our knowledge, this is the first comprehensive protein profiling of TMSC-derived sEV, demonstrating the presence of functional proteins and their capacity to regulate the MAPK and PI3K-AKT signaling pathways in the recipient TM cells. This highlights the potential of TMSC sEV in advancing cell-free therapeutic strategies for POAG in the future.
HostingRepository	PRIDE
AnnounceDate	2025-06-13
AnnouncementXML	Submission_2025-06-13_00:29:00.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iswarya R
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-18 00:25:09	ID requested
⏵ 1	2025-06-13 00:29:00	announced

Iswarya R, Maheshwari JJ, Krishnadas S, Vanniarajan A, Dharmalingam K, Priya CG, Proteome cargo and functional heterogeneity of extracellular vesicles derived from stem cells and differentiated cells of human trabecular meshwork. Mol Biol Rep, 52(1):564(2025) [pubmed]

10.1007/s11033-025-10663-4;

submitter keyword: proteomics, glaucoma, small extracellular vesicles,Trabecular meshwork stem cells

Gowri Priya Chidambaranathan
contact affiliation	Scientist, Department of Immunology and Stem Cell Biology, Aravind Medical Research Foundation, Madurai, Tamil Nadu, India.
contact email	gowri@aravind.org
lab head
Iswarya R
contact affiliation	Aravind Medical Research Foundation
contact email	aishukrish2020@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059934
     1. Label: PRIDE project
     2. Name: Small Extracellular Vesicles Derived from Human Trabecular Meshwork Stem Cells are distinct from TM Cells in their Proteome Cargo and Function