PXD059932

PXD059932 is an original dataset announced via ProteomeXchange.

Title	Urinary bacteriophage cooperation with bacterial pathogens during human urinary tract infections supports lysogenic phage therapy
Description	Despite much promise to overcome drug-resistant infections, clinical studies of bacteriophage anti-bacterial therapy have failed to show durable effectiveness. Although lysogeny plays an important role in bacterial physiology, its significance in diverse microbiomes remains under-studied. Here, we tested the hypotheses that 1) urinary microbiome phage populations switch to a higher relative proportion of temperate phages and 2) the activity of the phage recombination machinery (integration / excision / transposition) is higher during human urinary tract infections (UTIs) than in non-infected urinary tracts. Using human urine, model organisms, mass spectrometry, gene expression analysis, and the phage phenotype prediction model BACPHLIP, the results support our hypotheses at the functional protein and gene level. From a human health perspective, are temperate phages part of the problem and not the defenders we wished them to be? These data support the use of lysogenic phages as a therapeutic Trojan Horses.
HostingRepository	PRIDE
AnnounceDate	2025-01-17
AnnouncementXML	Submission_2025-01-17_15:47:07.221.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059932
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alessandra Luchini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-17 14:45:19	ID requested
⏵ 1	2025-01-17 15:47:07	announced

10.6019/PXD059932;

ProteomeXchange project tag: Kidney Urine (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Urinary tract infection, bacteriphages

Alessandra Luchini
contact affiliation	George Mason University
contact email	aluchini@gmu.edu
lab head
Alessandra Luchini
contact affiliation	George Mason University
contact email	aluchini@gmu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059932
     1. Label: PRIDE project
     2. Name: Urinary bacteriophage cooperation with bacterial pathogens during human urinary tract infections supports lysogenic phage therapy