PXD059930

PXD059930 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of experimental foodcrusts reveals discrepancies between food input and recovered protein signal
Description	In order to investigate how representative the recoverable proteins from charred organic residues (foodcrust) are of the food mixture cooked to produce them, a protein-rich (salmon), lipid-rich (beef fat) and carbohydrate-rich (beetroot) food were mixed, cooked and analysed. The three ingredients were mixed in 20% increments, resulting in 21 different mixtures covering all combinations of the three foodstuffs. The mixtures were placed in ceramic vessels and heated nine times with samples taken after the first, fifth and ninth heating event. Then the vessels with foodcrust were buried for 52 days and sampled again after excavation. The extracted proteins indicate substantial disparities in the relative abundance of an ingredient in the food mixture and the percentage of identified proteins it contributed. In particular salmon proteins seem overrepresented in the recovered proteome, followed by beef fat and lastly beetroot. Additionally, we found that only a limited number of proteins could be identified after the burial and that only salmon and beetroot contributed to the after burial proteomes.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_17:28:58.065.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD059930
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Dekker
SpeciesList	scientific name: blank sample; NCBI TaxID: 2582415; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Beta vulgaris (Sugar beet); NCBI TaxID: 161934; scientific name: Salmo salar (Atlantic salmon); NCBI TaxID: 8030;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-17 12:10:55	ID requested
⏵ 1	2025-08-24 17:28:58	announced

10.6019/PXD059930;

Dekker JAA, Hagan R, Collins M, Hendy J, An unclean slate, discrepancies between food input and recovered protein signal from experimental foodcrusts. PLoS One, 20(8):e0330195(2025) [pubmed]

10.1371/journal.pone.0330195;

submitter keyword: Organic residue, experimental archaeology, foodcrust, complex mixtures, metaproteomics

Matthew James Collins
contact affiliation	Section for Geobiology, Globe Institute, University of Copenhagen, Copenhagen, Denmark. McDonald Institute for Archaeological Research, University of Cambridge, UK.
contact email	matthew@palaeome.org
lab head
Jan Dekker
contact affiliation	University of York, UK, University of Copenhagen, Denmark
contact email	jan@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD059930

PRIDE project URI

• PRIDE
  1. PXD059930
     1. Label: PRIDE project
     2. Name: Proteomic analysis of experimental foodcrusts reveals discrepancies between food input and recovered protein signal