PXD059899

PXD059899 is an original dataset announced via ProteomeXchange.

Title	Global surface proteomes of hPSC-derived brain microvascular endothelial-like cells (hPSC-BMELCs), human cerebral microvascular endothelial cell line D3 (HCMEC/D3), and human umbilical vein endothelial cells (HUVECs)
Description	Abundance proteomics analysis was performed to characterize the surface proteome of three cell lines: hPSC-BMELCs, a stem cell derived model for human blood-brain barrier endothelial cells; HCMEC/D3, an immortalized cell line of brain microvascular endothelial cells; and HUVECs, primary human endothelial cells isolated from the umbilical vein. The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit was used which enables biotinylation and isolation of cell surface proteins by labeling with EZ-Link Sulfo-NHS-SS-Biotin, a thiol-cleavable amine-reactive biotinylation reagent, followed by capture with NeutrAvidin Agarose and reduction of the disulfide bond with DTT for protein release. Technical replicates of cells treated with biotin and without biotin were included for each cell line.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:30:37.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehdi Bouhaddou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-16 16:23:37	ID requested
⏵ 1	2025-11-09 16:30:38	announced

10.1016/J.CELREP.2025.116305;

submitter keyword: Abundance proteomics, receptors, endothelial cells, surface proteome

Mehdi Bouhaddou
contact affiliation	University of California Los Angeles
contact email	mehdibouhaddou@gmail.com
lab head
Mehdi Bouhaddou
contact affiliation	UCLA
contact email	bouhaddoulab@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD059899

PRIDE project URI

• PRIDE
  1. PXD059899
     1. Label: PRIDE project
     2. Name: Global surface proteomes of hPSC-derived brain microvascular endothelial-like cells (hPSC-BMELCs), human cerebral microvascular endothelial cell line D3 (HCMEC/D3), and human umbilical vein endothelial cells (HUVECs)