PXD059881

PXD059881 is an original dataset announced via ProteomeXchange.

Title	CIP2A mediates mitotic recruitment of SLX4/MUS81/XPF to replication stress-induced DNA lesions to maintain genome integrity
Description	Perturbed DNA replication can result in genomic regions that remain incompletely replicated at the point that cells enter mitosis. Transmission of replication-mediated DNA lesions into mitosis may prevent proper chromosomes segregation, and cells therefore require mechanisms to resolve these lesions during mitosis. CIP2A in conjunction with TOPBP1 has been described to function as a molecular tether to connect broken DNA molecules during mitosis, but a role for CIP2A in processing of incompletely replicated DNA has remained unclear. We find that the CIP2A-TOPBP1 complex forms large filamentous structures at sites of at sites of mitotic DNA synthesis during mitosis. Using endogenous IP we aimed to identify CIP2A-interactions induced by irradiation or replication inhibition, and find that CIP2A recruits members the SMX tri-nuclease complex, including SLX4, MUS81 and ERCC1/XPF.
HostingRepository	PRIDE
AnnounceDate	2025-10-24
AnnouncementXML	Submission_2025-10-24_05:13:50.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rolf de Boer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-16 06:55:13	ID requested
⏵ 1	2025-10-24 05:13:50	announced

10.1101/2025.04.03.647079;

submitter keyword: None

Marcel van
contact affiliation	Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713GZ, Groningen, the Netherlands (lab head)
contact email	m.vugt@umcg.nl
lab head
Rolf de Boer
contact affiliation	University Medical Center Groningen
contact email	h.r.de.boer@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD059881

PRIDE project URI

• PRIDE
  1. PXD059881
     1. Label: PRIDE project
     2. Name: CIP2A mediates mitotic recruitment of SLX4/MUS81/XPF to replication stress-induced DNA lesions to maintain genome integrity