PXD059871
PXD059871 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL |
Description | Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF Pro 2 mass spectrometer (Bruker Daltonics) in data-dependent mode with parallel accumulation serial fragmentation (PASEF) enabled. The raw data was analyzed using Fragpipe (v 22.0). MS/MS spectra were searched using MSFragger (v 4.1) against forward and reversed Homo sapiens UniProt sequence database (UP000005640) supplemented with common contaminants (total 20,979 sequences excluding decoys). |
HostingRepository | MassIVE |
AnnounceDate | 2025-06-05 |
AnnouncementXML | Submission_2025-06-05_21:16:44.532.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nikhil Dev Narendradev |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-01-16 02:32:50 | ID requested | |
⏵ 1 | 2025-06-05 21:16:44 | announced |
Publication List
no publication |
Keyword List
submitter keyword: RFFL, Interactome, E3 ligase, Substrates, DatasetType:Proteomics |
Contact List
Prof. S. Murty Srinivasula | |
---|---|
contact affiliation | Indian Institute of Science Education and Research Thiruvananthapuram |
contact email | sms@iisertvm.ac.in |
lab head | |
Nikhil Dev Narendradev | |
contact affiliation | Indian Institute of Science Education and Research Thiruvananthapuram |
contact email | nikhilndev18@iisertvm.ac.in |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000096868/ |