PXD059840 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Pervanadate mediated cysteine oxidation directly activates SRC kinase |
| Description | Protein tyrosine phosphorylation (pTyr) is regulated by protein tyrosine phosphatases (PTPs) and27 kinases, helping cells detect and react to environmental changes. Pervanadate is commonly used to increase pTyr levels by inhibiting PTPs, leading to the belief that PTPs are the main regulators of pTyr in cell culture. However, we found that pervanadate not only inhibits PTPs but also activates the SRC tyrosine kinase by oxidizing specific cysteine residues and promoting extensive conformational changes. Our study explains how cysteine oxidation activates SRC by overcoming kinase domain autoinhibition and altering phosphopeptide binding by its SH2 domain. We also show that these cysteine residues are essential for SRC to promote cellular overgrowth. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-05 |
| AnnouncementXML | Submission_2026-02-05_06:01:41.422.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Maxime Bourguet |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-01-15 14:55:26 | ID requested | |
| ⏵ 1 | 2026-02-05 06:01:41 | announced | |
Publication List
Keyword List
| submitter keyword: SRC, oxidation, kinase, HDX-MS |
Contact List
| Hayley J. Sharpe |
|---|
| contact affiliation | Signalling Programme, Babraham Institute, Cambridge, UK |
| contact email | hayley.sharpe@babraham.ac.uk |
| lab head | |
| Maxime Bourguet |
|---|
| contact affiliation | MRC Laboratory of Molecular Biology |
| contact email | mbourguet@mrc-lmb.cam.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD059840 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD059840
- Label: PRIDE project
- Name: Pervanadate mediated cysteine oxidation directly activates SRC kinase
